添加正十六烷对高山被孢霉发酵生产花生四烯酸的影响  

Influences of n-hexadecane on Mortierella alpine growth and arachidonic acid production

作　　者：彭超[1] 黄和[1,2] 刘欣[1] 肖爱华[2] 任路静[1] 

机构地区：[1]南京工业大学制药与生命科学学院,南京210009 [2]南京工业大学材料化学工程国家重点实验室,南京210009

出　　处：《食品科技》2010年第5期2-5,共4页Food Science and Technology

基　　金：国家重点基础研究发展计划项目(973计划)(2007CB707805);国家"863"项目(2006AA02Z240)

摘　　要：在高山被孢霉培养过程中,氧的供给是细胞生长和花生四烯酸合成的重要因素。通过添加正十六烷（作为氧载体）,有效改善发酵体系中的氧传递速率,从而促进了高山被孢霉合成花生四烯酸的能力。实验结果表明：添加正十六烷2%~4%（v/v）,花生四烯酸产量随正十六烷添加量逐步提高,最高时达到12.65g/L;当正十六烷添加量大于5%时,对花生四烯酸的合成表现出明显抑制作用;正十六烷的添加对细胞干质量有明显的促进作用,而对菌体中油脂含量及油脂中花生四烯酸的含量没有太大的影响。Oxygen supply played a major role for Mortierella alpina growth and arachidonic acid production.Two-phase fermentation system with the addition of n-hexadecane（oxygen vector） was applied to enhance oxygen transfer and promote the ability of arachidonic acid synthesis by Mortierella alpina.The results showed that higher arachidonic acid production was observed in shake flask containing 2%～4%（v/v） n-hexadecane,the maximum archidonic acid productivity reached 12.65 g/L.Whereas higher concentration of n-hexadecane showed somewhat inhibition to the Mortierella alpina growth and arachidonic acid formation.The results suggested that the the higher arachidonic acid yield did not resulted from the improvement of lipids content in biomass or the improvement of arachidonic acid content in lipids,but directly from the improvement of biomass of the Mortierella alpine.

关 键 词：花生四烯酸 高山被孢霉 氧载体 正十六烷 发酵 

分 类 号：TQ921[轻工技术与工程—发酵工程]