超滤法提取纳豆激酶的技术参数优化  

Study on technology for extracting nattokinase by ultrafiltration

作　　者：陈景鑫[1] 刘妍妍[1] 沙维[1] 张丽萍[1] 

机构地区：[1]黑龙江八一农垦大学食品学院,大庆163319

出　　处：《食品科技》2010年第5期225-229,共5页Food Science and Technology

基　　金：黑龙江省农垦总局课题(HNKXIV-06-05a)

摘　　要：目的:研究超滤系统中超滤压力、超滤温度、料液pH值主要参数对膜通量的影响,确定超滤分离纳豆激酶的最佳工艺条件。方法:以实验室制备的纳豆激酶粗酶液为原料,经超滤分离,层析分离确定纳豆激酶的比活力、纯化倍数及回收率。结果:最佳超滤压力为0.25MPa、料液温度为37℃、料液pH为7;可得到比活力为9610.46IU/mg、纯化倍数为2.36、回收率为92.3%的酶液,酶液经HPD-400树脂洗脱得到单一蛋白峰,经SDS-聚丙烯酰胺凝胶电泳,表现为单一蛋白条带,相对分子质量约为28000u。结论:采用超滤技术对纳豆激酶进行分离提纯,可提高酶活性及回收率,技术参数可行。Objective：Studyed the impact of membrane flux on utrafiltration system pressure,ultrafiltration temperature and pH,determined the optimum conditions of nattokinase separation.Methods：the raw materials was prepared from crude nattokinase liquid,by ultrafiltration and chromatography determined the specific activity,purification multiples and recovery rate.Results：The optimized conditions were as following：reaction pressure 0.25 MPa,liquid temperature 37 ℃,liquid pH 7,the specific activity 9610.46 IU/mg,the purification multiple 2.36 and the recovery rate 92.3%,the purified enzyme was demonstrated by SDS-PAGE to be a homogeneous protein,molecular mass was about 28000 u.Conclusion：It is feasible to separate and purify nattokinase by using ultrafiltration technology,and it can elevate activity and the recovery rate.

关 键 词：纳豆激酶 超滤 比活力 纯化倍数 回收率 

分 类 号：TQ925[轻工技术与工程—发酵工程]