食品致敏性评价啮齿类动物模型研究——C3H/HeJ小鼠动物模型  

Study on rodent model to detect food allergenicity——C3H/HeJ mice model

作　　者：孙拿拿[1] 张馨[1] 崔文明[1] 王伟[1] 张文众[1] 彭于发[2] 李宁[1] 贾旭东[1] 

机构地区：[1]中国疾病预防控制中心营养与食品安全所,北京100050 [2]中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室

出　　处：《卫生研究》2010年第3期310-312,共3页Journal of Hygiene Research

基　　金：国家科技部973课题(No.2007CB109207);转基因重大专项(No.2008ZX08011-005)

摘　　要：目的研究C3H/HeJ小鼠作为食品致敏性评价动物模型的可行性,探讨不同致敏途径适当的致敏周期以及辅助性T淋巴细胞因子的变化。方法通过经口灌胃及腹腔注射两种途径给予雌性C3H/HeJ小鼠致敏蛋白——卵清蛋白(OVA),共35天。分别在第14、28、35天内眦取血分离血清,测定OVA特异性IgE。于第35天取脾脏,荧光实时定量PCR检测Th2型细胞因子IL-4及Th1型细胞因子IFN-γ的mRNA表达水平。结果与阴性对照组相比,经口灌胃OVA组小鼠第28和35天血清中OVA特异性IgE浓度显著升高(P<0.05);腹腔注射OVA+Al(OH)3组小鼠第14、28和35天血清中OVA特异性IgE浓度显著升高(P<0.05)。与阴性对照组相比,OVA组与OVA+Al(OH)3组小鼠细胞因子IL-4的表达水平均显著升高;细胞因子IFN-γ的mRNA表达水平均显著降低。结论C3H/HeJ小鼠可以作为食品致敏性评价动物模型,经口灌胃途径及腹腔注射途径的最佳致敏周期分别为14天和28天。Objective To study the feasibility of C3H /HeJ mice as the food allergenicity rodent model and explore the appropriate period of two different routes of exposure and the change of helper T lymphocyte cell.Methods Allergenic protein ovalbumin（OVA） was administered intraperitoneally or orally to female C3H /HeJ mice for 35days.Blood samples were taken on the 14th,28th and 35th days from the orbital plexus and sera were obtained to determine OVA specific IgE（OVA sIgE）.Spleen samples were obtained on the 35th day to detect the mRNA expression of Th2-type cytokines Il-4 and Th1-type cytokines IFN-γ by real-time RT-PCR.Results Compared with the control group,the concentration of OVA sIgE on 28th and 35th days were significantly increased in OVA group by gavage（P 0.05）.The concentration of OVA sIgE on 14th,28th and 35th days were significantly higher than the control group when OVA was given intraperitoneally（P 0.05）.Compared with the control group,the mRNA expression of Il-4 of OVA group and OVA ＋ Al（OH）3 group were significantly increased.The mRNA expression of IFN-γ of OVA group and OVA ＋ Al（OH）3 group were significantly lower than the control group.Conclusion C3H /HeJ mice might be used as a rodent model for the assessment of allergenicity of food.The appropriate period is the 14th day for the route of intraperitoneal injection and the 28th day for oral gavage,respectively.

关 键 词：食品致敏性 动物模型 C3H/HeJ小鼠 细胞因子 

分 类 号：R155.31[医药卫生—营养与食品卫生学] TS201.6[医药卫生—公共卫生与预防医学]