多元线性回归设计优化热变性法提取猪血SOD的最佳条件研究  

Best Condition on Purifying Superoxide Dismutase(SOD) in Swine Blood by Heating Denaturation Method Based on the Design of Multiple Linear Regression

作　　者：刘静波[1] 刘婧[1] 张杰[1] 李洪山[1] 赵娅娅[1] 安晓宁[1] 潘风光[1] 

机构地区：[1]吉林大学军需科技学院,营养与功能食品研究室,吉林长春130062

出　　处：《食品研究与开发》2010年第5期19-22,共4页Food Research and Development

基　　金：长春市科技局科技支撑计划项目(08KZ26)

摘　　要：为了开发利用猪血资源,以猪血为原料,采用多元线性回归研究热变性法提取猪血SOD的最佳工艺条件。结果表明:热变性法具有操作简单,成本低廉,无污染等优点。试验建立加热时间、加热温度和硫酸铜浓度对SOD的活性和蛋白质含量影响的优化数学回归模型,获得提取SOD的最佳工艺参数:加热时间为20 min,加热温度为55℃,硫酸铜浓度为3.5%。In order to develop the swine blood,this experiment taked the swine blood as a raw material and optimize the heating denatureation process of SOD from the swine blood.Results showed that the heating denatureation method was simple,low-cost and no pollution.The mathematical model of the effects of heating time,heating temperature and copper sulfate concentration on enzyme activity and protein content was established.The optimal conditions were determined as： heating time 20 min,heating temperature 55 ℃,copper sulfate concentration 3.5 %.

关 键 词：猪血 SOD 热变性 多元线性回归 

分 类 号：TS251.93[轻工技术与工程—农产品加工及贮藏工程]