溶氧对Blakeslea trispora分批发酵生产β-胡萝卜素的影响  

Effect of Dissolved Oxygen on Batch Fermentation to Produce β-carotene by Blakeslea trispora

作　　者：张宁[1] 虞龙[2] 

机构地区：[1]中国林业科学研究院林产化学工业研究所国家林业局林产化学工程重点开放性实验室,江苏南京210042 [2]南京工业大学,江苏南京210042

出　　处：《微生物学杂志》2009年第6期32-35,共4页Journal of Microbiology

摘　　要：在摇瓶和5 L发酵罐中研究了溶氧(DO)对Blakeslea trispora分批发酵生产β-胡萝卜素的影响,总结了5 L发酵罐中β-胡萝卜素发酵过程中溶氧的变化规律。结果表明,当500 mL摇瓶装液量为50 mL,转速为240 r/min条件下发酵生产β-胡萝卜素产量最大,达到3.416 g/L;5 L发酵罐中,在搅拌转速为1 000 r/min,通气量为1.5 vvm的条件下,β-胡萝卜素的产量可达到3.712 g/L,略高于摇瓶,这可能是由于5 L发酵罐中的气液传递和混合状况好于摇瓶,促进了产物的合成。The effect of dissolved oxygen（DO） on β-carotene batch fermentation by Blakeslea trispora in shake flasks and 5 liters stirred fermentor were investigated and the fermentation laws of β-carotene in 5 liters stirred fermentor were concluded.It was shown that with the culture volume of 50 mL in 500 mL shake flasks and a 240 rpm shaking speed,the yield of β-carotene was 3.416 g/L.In 5 liters stirred fermentor,under the condition of 1 000 rpm agitation speeds and 1.5 vvm aeration rate,the yield of β-carotene was 3.712 g/L,and was higher than in shaker flasks.This may be due to the conditions of gas-liquid transferring and mixing in 5 liters stirred fermentor were better than in shaker flasks,and promoted the synthesis of β-carotene.

关 键 词：Β-胡萝卜素 溶氧 分批发酵 

分 类 号：TQ922[轻工技术与工程—发酵工程]