固定化谷氨酸脱羧酶性能的研究  

STUDY ON THE CHARACTERISTICS OF IMMOBILIZED L-GLUTAMIC DECARBOXYLASE ON CM-CADB RESIN

作　　者：吴国琪[1] 凌达仁[1] 王忱[1] 王钒[1] 宋国强[1] 

机构地区：[1]江苏石油化工学院应用化学系,常州213016

出　　处：《离子交换与吸附》1999年第1期71-76,共6页Ion Exchange and Adsorption

基　　金：江苏省自然科学基金

摘　　要：本文首次用羧甲基化的以N，N’-甲叉双丙烯酰胺交联的烯丙基葡聚糖（简称CM-CADB）凝胶生化树脂为新型载体，将谷氨酸脱羧酶固定在CM-CADB树脂上。研究了固定化GDC的活性与低物浓度、pH、温度的依存性；动态响应特性；热稳定性和寿命；求算了米氏常数和反应活化能；将固定化GDC酶柱与进样系统-离子活度分析器-计算机数据采集系统匹配；构成酶传感器谷氨酸检测装置，测定了固定化GDC酶柱的能斯特线性响应曲线，线性方程为y=43．3x＋181．6；线性相关系数为0．99。The immobilized L-glutamic decarboxylase (GDC) on the CM-CADB (carboxymethyl-copolymer of allyl dextran and N,N'-methylene-bisacrylamide) ion exchange resin has been studied in this paper. The dependence of the activity of immobilized GDC on the substrate concentration, pH, temperature were studied. The dynamic response to L-Glutamic acid, the thermostability and the longevity were investigated. The constant of Michaelis and the activation energy were calculated. The enzyme column of immobilized GDC matching with the flowinjection-ion analyzer-data collection make up the original form of the apparatus of biosensor measuring L-glutamic acid. The Nernst linear response curve has been measured.

关 键 词：固定化酶 谷氨酸脱羧酶 测定 味精 

分 类 号：TS264.23[轻工技术与工程—发酵工程]