大曲中放线菌和青霉产酶初探  

Enzymatic Characteristic Preliminary of Actinomycete and Penicillium Isolated from Daqu

作　　者：刘鸿[1] 方尚玲[1] 陈茂彬[1] 李锐利[1] 

机构地区：[1]发酵工程省部共建教育部重点实验室,湖北工业大学生物工程学院,湖北武汉430068

出　　处：《酿酒》2010年第2期38-41,共4页Liquor Making

基　　金：湖北省自然科学基金项目(2008CDB063);湖北工业大学博士科研启动基金项目(BSQD0910)

摘　　要：以从大曲和糟醅中分离出的放线菌和青霉为研究对象,用淀粉培养基和纤维素刚果红培养基对两种菌分别进行初筛,根据透明圈和菌落直径之比大小筛选出产淀粉和纤维素酶的菌株,进一步通过固体发酵培养并测定淀粉酶和纤维素酶活,筛选出三株分解淀粉和纤维素的菌株命名为放线菌F1,青霉Q4,青霉Q5,其中青霉Q4的两种酶活力都较高,α-淀粉酶活达到1160u/g干曲,内切纤维素酶活达到301.83u/g干曲,滤纸酶活达到48.26u/g干曲。Actinomycete and Penicillium were isolated from Daqu and Zhaopei .Theses strains were firstly screened with the Congo red cellulose identification culture medium and the solid medium using soluble starch as substrate .We selected strains with clear zones and measured HC value of the stains（ratio of diameter of clearing zones and colony） . Secondly these cellulose-producing and a-amylase-producing strains were fermented on solid medium and measured enzyme solution carboxymethyl cellulose（CMC） activity,filter paper activity（FPA） and a-amylase activity. Finally three cellulose and amylase decomposing strains were screened and individually named F1 belonged to Actinomycete,Q4 and Q5 belonged to Penicillium. And the strain Q4 with the highest activity of cellulose and a-amylase,which had α-amylase activity of 1160u/g dry medium,CMC activity of 301.83 u/g dry medium and FPA of 48.26 u/g dry medium.

关 键 词：放线菌 青霉 Α-淀粉酶 纤维素酶 

分 类 号：TS262.3[轻工技术与工程—发酵工程] TS261.11[轻工技术与工程—食品科学与工程]