还原糖测定方法的比较研究  

Comparative study on the determination of reducing sugar

作　　者：张永勤[1] 王哲平[1] 宋雨梅[1] 栾东磊[2] 薛长湖[2] 

机构地区：[1]青岛科技大学化工学院,山东青岛266042 [2]中国海洋大学食品科学与工程学院,山东青岛266003

出　　处：《食品工业科技》2010年第6期321-323,326,共4页Science and Technology of Food Industry

摘　　要：采用3-甲基-2-苯并噻唑酮腙(MBTH)法、3,5-二硝基水杨酸(DNS)法和铁氰化钾法分别测定了葡萄糖、木糖、岩藻、阿拉伯糖、N-乙酰氨基葡萄糖、氨基葡萄糖六种还原糖。通过对测定结果的比较分析表明:MBTH法测还原糖的精密度和回收率与DNS法和铁氰化钾法相近;MBTH法测还原糖的灵敏度最高,铁氰化钾法次之,DNS法最低。因此,MBTH法特别适合还原糖的微量测定。Glucose, Xylose, Fucose, Arabinose, N- acetylglucosamine methods of 3- Methyl- 2- benzoth ferricyanide. The results indicated azolinonehydrazone （ MBTH）, 3,5 and Glucosamine were determined by the dinitrosalicylic acid （DNS） and potassium that all the methods were with high precision and recovery rate, the MBTH method had the highest sensitivity, followed by the potassium ferricyanide method, the lowest was the DNS method.So the MBTH method was particularly suitable to the micro-determination of reducing sugar.

关 键 词：还原糖 3-甲基-2-苯并噻唑酮腙(MBTH) 3  5-二硝基水杨酸(DNS) 铁氰化钾 

分 类 号：TS207.3[轻工技术与工程—食品科学]