发酵程度对普洱茶活体抗氧化作用的影响  

Influence of Pile-Fermentation Degree on the Antioxidative Activity of Pu-erh Tea in Vivo

作　　者：张灵枝[1] 陈维信[2] 王登良[1] 谭新东[1] 郭剑英[3] 

机构地区：[1]华南农业大学茶业科学研究所,广东广州510642 [2]华南农业大学广东省果蔬保鲜重点实验室,广东广州510642 [3]华南农业大学兽医学院,广东广州510642

出　　处：《茶叶科学》2010年第3期208-212,共5页Journal of Tea Science

基　　金：国家自然科学基金(30700554)

摘　　要：以晒青毛茶及不同渥堆程度普洱茶饲喂实验小鼠,研究小鼠肝组织抗氧化酶活性变化规律。结果表明,各处理小鼠,其肝组织抑制羟自由基能力、SOD活力、GSH-PX活力变化规律基本一致,各饲喂茶样组以上三指标均远远高于对照(基础饲料饲喂),并以晒青毛茶处理组最高,随饲喂茶样渥堆程度增加,以上三指标逐渐降低。各处理小鼠肝组织MDA含量与其SOD活力高度负相关,相关系数r=-0.942;晒青毛茶处理组SOD活力最强,MDA含量最低;与对照相比,各茶样处理组MDA含量均极显著下降(P<0.01)。各茶样处理小鼠肝组织中CAT活力均高于对照组,除第4次翻堆样处理外,其他处理与对照之间差异达显著水平(P<0.05)。各茶样处理组T-AOC均极显著高于对照组(P<0.01),以晒青毛茶处理组最强,随着渥堆程度增加,T-AOC呈下降趋势。研究结果说明,普洱茶渥堆发酵工序对其抗氧化作用有重要的影响。Antioxidative enzymes activity in the livers of the mice fed with the sun-dried green tea and the Pu-erh tea with different pile-fermentation degree were studied. Results were as follows： compared with the control group （basic feed group）, the hydroxyl radical-scavenging activity, glutathione peroxidase （GSH-PX） activity and superoxide dismutase （SOD） activity in the livers of the mice fed with different tea samples were significantly increased（P0.01）, but decreased with the extents of pile-fermentation degree of Pu-erh tea increasing. This increase was the highest in the sun-dried green tea treatment. Of all the treatments, there was a significant negative correlation between the maleic dialdehyde （MDA） content and the SOD activity and the correlative coefficient was -0.942. The SOD activity was the highest while the MDA content was the lowest in the sun-dried green tea treatment （P〈0.01）. The MDA content of other treatments were highly decreased when compared with that of the control （P〈0.01）. Compared with the control, catalaes （CAT） activity were highly increased except that of the fourth pile-fermentation tea treatment （P0.01）. As to the total anti-oxidation capacity （T-AOC）, it was significantly increased compared with that of the control （P〈0.01）, but decreased with the extents of pile-fermentation degree increasing. The sun-dried green tea treatment exhibited the strongest T-AOC （P〈0.01）. The study showed that the pile-fermentation process exerted significant effects on the antioxidative activity of Pu-erh tea.

关 键 词：普洱茶 渥堆 抗氧化酶 总抗氧化能力 丙二醛 

分 类 号：R151[医药卫生—营养与食品卫生学]