非水相酶促不可逆转酯反应合成阿魏酸三油酸甘油酯  

Enzymatic Synthesis of Feruloyl-oleylglycerol through Irreversible Transesterification in Nonaqueous Phase

作　　者：於洋[1] 郑妍[1] 权静[1] 朱利民[1] 

机构地区：[1]东华大学化学化工与生物工程学院,上海201620

出　　处：《精细化工》2010年第5期471-475,共5页Fine Chemicals

基　　金：国家自然科学基金资助项目(No.507730009);中国教育部药用纺织材料"111工程"资助项目(No.B07024);上海市科委重点学科基金资助项目(08JC1400600);中央高校基本科研业务费专项基金资助项目~~

摘　　要：在有机相甲苯中通过阿魏酸乙烯酯(VF)和三油酸甘油酯(TO)的酶促转酯反应合成油脂抗氧化剂阿魏酸三油酸甘油酯。用阿魏酸乙烯酯作为底物可使转酯反应不可逆,这有利于产物产率的提高并缩短反应时间。通过核磁共振波谱和质谱对阿魏酸乙烯酯的结构进行了表征,并考察了底物摩尔比、反应时间、反应温度、酶用量、水活度对产物产率的影响。结果表明,以甲苯为溶剂,当底物n(VF)∶n(TO)=1∶3,反应时间62 h,反应温度55℃、酶用量20 g/L,水活度(aw)为0.07时,产物的产率最大,达96.73%。The synthesis of a new-type antioxidant for feruloyl-oleylglycerol through the lipase-catalyzed irreversible transesterification of vinyl ferulate（VF） and triolein（TO） was studied.Vinyl ferulate made the transesterification reaction irreversible.The structure of VF was identified by NMR and MS and the influential factors of substrate molar ratio,reaction time,reaction temperature,enzyme amount and water activity were systematically investigated.Results indicated that in the toluene reaction system the optimal conditions are as follows：n（VF）∶n（TO）=1∶3,reaction time 62 h,reaction temperature 55 ℃,enzyme amount 20 g/L and water activity（aw） 0.07.Under the optimal conditions,the maximum yield was obtained as high as 96.73%.

关 键 词：阿魏酸三油酸甘油酯 阿魏酸乙烯酯 不可逆转酯反应 脂肪酶 非水相 食品用化学品 

分 类 号：TQ925.6[轻工技术与工程—发酵工程]