青霉PT95菌株固态发酵产生类胡萝卜素的研究  

SOLID STATE FERMENTATION WITH PENICILLIUM SP. PT95 FOR CAROTENOID PRODUCTION

作　　者：韩建荣[1] 徐军[1] 

机构地区：[1]山西大学生命科学系

出　　处：《微生物学报》1999年第2期148-153,共6页Acta Microbiologica Sinica

基　　金：山西省教委基金

摘　　要：本文对青霉Ｐｅｎｉｃｉｌｉｕｍｓｐ．ＰＴ９５菌株在固态发酵条件下菌核内产生类胡萝卜素进行了初步研究。结果表明，在３种固态发酵培养基中，玉米粉培养基（ＳＭＡ）比麸皮培养基和棉籽壳培养基更适合于ＰＴ９５菌株固态发酵产生类胡萝卜素。为了增加菌核干重和提高类胡萝卜素产率，ＳＭＡ中需要添加氮源、碳源和植物油。在所试的各种氮、碳源中，以硝酸钠和麦芽糖效果最佳。通过正交试验确定了在培养基盐溶液中添加硝酸钠３ｇ／Ｌ，麦芽糖１０ｇ／Ｌ，豆油２５ｇ／Ｌ能使菌核干重由５３６ｇ／１００ｇ提高到９７０ｇ／１００ｇ（干料）；类胡萝卜素产率由２１４９μｇ／１００ｇ提高到５２６０μｇ／１００ｇ（干料）；β胡萝卜素在类胡萝卜素中的含量由６１４％提高到７１３％。A preliminary study on solid state fermentation (SSF) with Penicillium sp PT95 for carotenoid production was performed.The results showed that the production of carotenoid in sclerotia of PT95 was more efficient in corn meal medium than in either wheat bran medium or cottonseed hull medium.Addition of nitrogen and carbon sources as well as vegetable oil to media was required for increasing the dry weight of sclerotia and carotenoid yield.Among several tested compounds for nitrogen and carbon sources,sodium nitrate and maltose were the best.Through orthogonal experiments,the optimum culture medium was obtained by supplement of NaNO 3 3g,maltose 10g,soybean oil 2.5g to per liter of salt solution.Under the optimum culture conditions,the sclerotia dry weight increased from 5.36g to 9.70g per 100g dry substrate,the carotenoid yield from 2149 μg to 5260μg per 100g dry substrate,the proportion of β carotene in carotenoids from 61.4% to 71.3%.

关 键 词：青霉 菌核 固态发酵 类胡萝卜素 Β-胡萝卜素 

分 类 号：Q939[生物学—微生物学]