微波诱变结合化学诱变选育酸性蛋白酶高产菌  

STUDIES ON THE SCREENING HIGH YIELD ACID PROTEASE PRODUCING STRAIN L336 BY COMBINING MICROWAVE IRRADIATION WITH CHEMICAL INDUCING

作　　者：李永泉[1] 翁醒华[1] 贺筱蓉[1] 

机构地区：[1]浙江大学生命科学学院

出　　处：《微生物学报》1999年第2期181-184,共4页Acta Microbiologica Sinica

基　　金：浙江省教育委员会资助

摘　　要：酸性蛋白酶是酶制剂工业比较重要的酶种，广泛应用于饲料、食品、皮革、医药和酿造工业中［１～４］，具有较大的市场潜力。本研究以宇佐美曲霉（Ａｓｐｅｒｇｉｌｕｓｕｓａｍｉ）白色突变株Ｂ１为出发菌株，通过微波诱变和亚硝基胍、硫酸锂复合诱变剂点试平板法诱变，选...A high yield acid protease producing strain Aspergrillus usamii L336 was screened from its parent strain B 1 by combining microwave irradiation with inducing of nitrosoguanidine and Li 2SO 4. By incubation of L336 in shaking flask at 31℃ for 88 h, its enzyme activity was 5500u/mL , and its properties for high yield producing acid protease remained stable after many times of subculture and storage for two months.

关 键 词：微波诱变 化学诱变 宇佐美曲霉 酸性蛋白酶 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]