低温生淀粉糖化酶菌株RS01分离及其酶学性质  

Characterization of Cold-active Raw Starch-digesting Glucoamylase from Isolated Strain RS01

作　　者：孙子羽[1] 迟乃玉[1] 王宇[2] 李兵[1] 张庆芳[1] 

机构地区：[1]大连大学生物工程学院辽宁省海洋微生物生物工程技术研究中心,辽宁大连116622 [2]大连工业大学生物与食品工程学院,辽宁大连116034

出　　处：《微生物学通报》2010年第6期798-802,共5页Microbiology China

基　　金：国家863计划项目(No2007AA021306)

摘　　要：自渤海湾海泥中分离得到一株产低温生淀粉糖化酶能力较强的菌株RS01,经形态学、生理生化特性及16S rRNA分析将其鉴定为气单胞菌属。对该菌株产的低温生淀粉糖化酶酶学性质进行初步研究,结果表明其最适酶反应温度为30℃,酶的热稳定性比较差,最适pH为5.4,经TLC鉴定酶解产物中有葡萄糖,表明该分离菌株具有产低温生淀粉糖化酶的能力。A strain producing cold-active raw starch-digesting glucoamylase was isolated from Bohai Bay＇ samples.The strain RS01 was identified as Aeromonas sp.based on the morphological,physiological characteristics and 16S rRNA sequence.The foundation on the enzymatic properties of partially purified Cold-active RSGA explained that the maximum activity of the enzyme was exhibited at 30℃ and pH 5.4.The enzyme thermal stability was poor.The glucose was identified by the TLC,which proved that the strain RS01 has the ability to produce cold-active raw starch-digesting glucoamylase.

关 键 词：低温生淀粉糖化酶 气单胞菌 酶学性质 

分 类 号：TQ925[轻工技术与工程—发酵工程]