碱性脂肪酶高产菌株的筛选及产酶条件的优化  

Screening of a High-Yielding Lipase-Producing Bacteria Strain and Optimization of the Conditions for Its Lipase Production

作　　者：王松[1] 李长春[1] 徐榕敏[1] 邱慧[1] 唐云明[1] 

机构地区：[1]西南大学生命科学学院,重庆400715

出　　处：《西南大学学报（自然科学版）》2010年第6期99-103,共5页Journal of Southwest University(Natural Science Edition)

基　　金：重庆市科委攻关项目资助项目(CSCT;2004AC1012)

摘　　要：从富含油脂的土样中采集原始菌种,通过富集培养、平板筛选和复筛等方法,获得脂肪酶产生菌.然后进行逐个因子实验,对产酶条件进行优化,得一株产脂肪酶达95U/mL的高产菌株.Original bacteria were collected from lipid-rich soil samples. Lipase-producing bacteria were obtained by enrichment culture, plate screening and repeated screening. Then factor experiments were conducted to optimize the culture conditions for lipase production. A high-yielding strain with a yield of 95 U/mL, Serratia sp. SL-11, was obtained.

关 键 词：脂肪酶菌株 筛选 优化 

分 类 号：Q55[生物学—生物化学]