154例t(8;21)急性髓系白血病遗传学分析  

Cytogenetic analysis of 154 case of acute myeloid leukemia with t(8;21)

作　　者：牧启田[1,2] 陈志妹[1,2] 楼基余[1,2] 程译帜[1,2] 王云贵[1,2] 倪万茂[1,2] 王焕萍[1,2] 徐欢[1,2] 俞运彪[1,2] 金洁[1,2] 

机构地区：[1]浙江大学血液病研究所 [2]浙江大学医学院附属第一医院血液科浙江省血液肿瘤(诊治)重点实验室,浙江杭州310003

出　　处：《浙江大学学报（医学版）》2010年第3期236-240,共5页Journal of Zhejiang University(Medical Sciences)

基　　金：国家高技术研究发展计划(863计划)重大课题子课题(2006AA02A405)

摘　　要：目的:探讨t(8;21)急性髓系白血病(acute myeloid leukemia,AML)的遗传学特点。方法:回顾性分析浙江大学医学院第一附属医院血液科154例t(8;21)AML的遗传学、免疫学和分子生物学资料。为便于分析染色体核型与FAB分型的关系,把患者分为单纯t(8;21)组(69例)、单纯伴性染色体丢失组(54例)和其他附加染色体组(31例)。结果:按FAB分型:M2127例(82.5%)、M515例(9.7%)、M46例(3.9%)、M14例(2.6%)、M02例(1.3%);附加染色体异常85例(55.2%),其中-Y43例,占男性核型的44.1%;-X 17例,占女性核型的27.9%;9q-9例,占总例数5.8%;+8 5例,占3.4%,+4 3例,占2.0%;其他类型染色体异常17例,占11.4%;其他附加染色体异常组有非M2(M0、M1、M4、和M5)病例11例,占该组35.5%,高于单纯t(8;21)组的17.4%,差异有统计学意义(P<0.05);伴性单纯染色体丢失组有4例(7.4%),低于单纯t(8;21)组,差异无统计学意义(P>0.05)。结论:t(8;21)AML常伴有其他染色体异常,主要见于M2型,除性染色体丢失以外附加染色体异常更易见于非M2型AML。Objective： To investigate the cytogenetic features of acute myeloid leukemia（AML） with t（8;21）.Methods： The clinical characteristics of 154 cases of acute myeloid leukemia with t（8;21） in our hospital were analyzed retrospectively.According to the chromosome karyotype,all cases were divided into three groups：the group without additional chromosome abnormality,the group with single sex chromosome loss and the group with additional chromosome abnormalities other than sex chromosome loss.Results： In this study,according to FAB classification,there were 127 cases of M2（82.5%）,15 of M5（9.7%）,6 of M4（3.9%）,4 of M1（2.6%） and 2 of M0（1.3%）.Cytogenetically,85（55.2%） AML patients with t（8;21） had additional chromosome abnormalities.The most common abnormalities were sex chromosome loss,of which-Y was detected in 44.1% of the male karyotype and X in 27.9%.Beside that,there were 9 cases of 9q-（5.8%）,5 of ＋8（3.3%）,3 of ＋4（2.0%） and 17 of other chromosome anomalies（11.4%）.In the group of t（8;21） with additional chromosome abnormalities,11 cases（35.5%） were non-M2 AML,higher than that in single t（8;21） group（17.4%）（P0.05）;however,there was no significant difference between the group of single t（8;21） and the group of t（8;21） with single sex chromosome loss（P〉0.05）.Conclusion： t（8;21） translocation is usually companied by additional chromosome abnormalities,particularly in M2;while t（8;21） with additional chromosome abnormalities other than sex chromosome loss is more frequently observed in non-M2 AML.

关 键 词：白血病 粒细胞 急性 染色体畸变 细胞遗传学 

分 类 号：R733.71[医药卫生—肿瘤]