阿魏酸酯酶产生菌的培养条件优化  

Optimization of Fermentation Conditions of Ferulic Acid Esterase Producers

作　　者：范韵敏[1] 李夏兰[1] 方柏山[1] 

机构地区：[1]华侨大学工业生物技术福建省高校重点实验室,福建泉州362021

出　　处：《华侨大学学报（自然科学版）》2010年第4期426-429,共4页Journal of Huaqiao University(Natural Science)

基　　金：国家重点基础研究发展(973)计划项目(2007CB707804)

摘　　要：通过均匀设计法和二次多项式逐步回归,对产阿魏酸酯酶的培养基配方进行优化,得出优化的培养基配方(质量分数):0.30%KH2PO4,0.60%Na2HPO4.7H2O,0.01%NaCl,0.80%酵母粉,1.33%麦糟.在此基础上,用单因素法考察发酵条件对产酶的影响,确定最佳条件:初始pH值为6.0,培养温度为28℃,摇床转速为210 r.min-1,发酵时间为68 h,装液量为75 mL,接种量为5%,麦糟粒径为0.054 mm.采用优化的培养基组分进行最优发酵培养,优化后的阿魏酸酯酶酶活达到119.36μkat.L-1,比优化前提高了32倍.The optimal parameters of fermentation conditions of ferulic acid esterase from brewer＇s spent grain were obtained through uniform-design and quadratic polynomial regression techniques.The optimal culture medium were as follows（%）：KH2PO4 0.30,Na2HPO4·7H2O 0.60,NaCl 0.01,yeast powder 0.80,brewer＇s spent grain 1.33.Based on the results above,the influence of fermentation conditions on enzymes were also studied by single factor method and the optimal conditions were confirmed as follows： the optimization initial pH 6.0,cultural temperature 28 ℃,the strain was cultivated with 75 mL medium in a 250 mL flask for 68 h on 210 r·min-1 shaker,inoculum concentration 5% and grain diameter 0.054 mm.Ferulic acid esterase reached 119.36 μkat·L-1 under optimal conditions,increased 32 times than that before optimization.

关 键 词：阿魏酸酯酶 麦糟 优化 均匀设计 

分 类 号：TQ920.6[轻工技术与工程—发酵工程] Q814.9[生物学—生物工程]