双波长比值光谱法测定饮料中的色素  

Dual-wavelength Ratio Spectrometric Determination of Carmine, Sunset Yellow and Tartrazine in Beverages

作　　者：杜建中[1] 李青梅[1] 朱艺强[1] 丁玎[2,3] 谭永辉[1] 

机构地区：[1]湛江师范学院广东高校新材料工程技术开发中心,广东湛江524048 [2]圣地亚哥州立大学公共卫生学院,美国圣地亚哥92123 [3]加州大学圣地亚哥分校预防医学系,美国圣地亚哥92137

出　　处：《食品科学》2010年第10期259-262,共4页Food Science

基　　金：湛江师范学院科学研究基金资助项目(L0514)

摘　　要：应用双波长比值光谱法测定了果味汽水、水果泡泡中胭脂红、日落黄、酒石黄的含量。依据该3种色素的比值光谱特征,以日落黄为干扰组分,分别选择431、456nm和509、525nm为测定酒石黄和胭脂红的波长;以胭脂红为干扰组分时,选择514、535nm为测定日落黄的波长。结果显示,酒石黄在0.1～60mg/L、胭脂红在0.2～80mg/L、日落黄在0.8～90mg/L的质量浓度范围内线性关系良好,平均回收率分别是100.5%、101.1%和102.4%。本方法操作简便、易于推广,可应用于实际样品的检测。A dual-wavelength ratio spectrometric method was presented for the determination of carmine, sunset yellow and tartrazine in fruit-flavored carbonated beverages. According to the spectral characteristics of the pigments, the wavelengths were chosen to be 431 nm and 456 nm for tartrazine detection and 509 nm and 525nm for carmine detection under the disturbance of sunset yellow and 514 nm and 535 nm for sunset yellow detection under the disturbance of carmine. Results showed that the developed standard curves for determining carmine, sunset yellow and tartrazine all exhibited good linearity over the concentration ranges of 0.2 to 80, 0.8 to 90 mg/L and 0.1 to 60 mg/L, respectively. The mean recoveries of 5 replicates of carmine, sunset yellow and tartrazine in orange-flavored Miranda soft drink were 100.5%, 101.1% and 102.4% respectively. This method proved to be simple and convenient and thus deserves to be popularized.

关 键 词：双波长比值法 胭脂红 日落黄 酒石黄 饮料 

分 类 号：O658.2[理学—分析化学]