高压脉冲电场促进啤酒酵母细胞RNA快速溶出的技术  

Fast dissolution of RNA from waste brewing yeast by high-voltage pulsed electric fields

作　　者：刘静波[1] 于一丁[1] 王睦[1] 林松毅[1] 王琴[1] 高丽霞[1] 孙鹏[1] 

机构地区：[1]吉林大学军需科技学院营养与功能食品研究室,长春130062

出　　处：《吉林大学学报（工学版）》2010年第4期1171-1176,共6页Journal of Jilin University:Engineering and Technology Edition

基　　金：吉林省科技厅农业重点项目(20070201)

摘　　要：以啤酒废酵母泥为原料,以啤酒废酵母细胞中的RNA溶出率为衡量指标,对比分析了高压脉冲电场(HPEF)技术对啤酒废弃酵母中RNA在水、乙醇、三氯乙酸3种提取溶剂中的溶出情况,并借助二次通用旋转组合实验设计,构建出HPEF快速提取啤酒废酵母细胞中的RNA的数学模型;对比发现,以乙醇为提取溶剂的RNA得率是水的1.69倍,RNA得率提高了40.86%,进而优化出HPEF提取RNA的最佳工艺参数为:以体积分数50%的乙醇为提取溶剂,电场强度为50kV/cm,脉冲数为2个(电场作用时间仅为0.0067s),液料比为30∶1,且在该条件下溶出的RNA得率达到0.85%。RNA from waste Brewer′s yeast was studied as raw material to analysis the effect of HPEF on the dissolution condition of RNA by water, ethanol, and TCA as extraction solvent, respectively. The mathematical model of RNA rapid extraction from waste Brewer′s yeast by HPEF was made use of twice general purpose rotation battery testing design. The mathematical model was given. The RNA yield by ethanol as extraction solvent was 1. 69 times of it by water. The RNA yield enhanced 40. 86% . The best parameters of extraction RNA from waste Brewer′s yeast by HPEF were ethanol as extraction solvent,50 kV/cm as electric field strength,2 as pulse number（ 0. 0067s as pulse time） ,liquid-solid ratio as 30∶ 1,in which condition that the RNA yield reached to 0. 85% .

关 键 词：食品加工技术 高压脉冲电场 RNA 快速溶出 

分 类 号：X792[环境科学与工程—环境工程]