转ChIFN-γ基因烟草抗虫机制研究  

A study on the insect-resistant mechanism of ChIFN-γ transgenic tobacco

作　　者：吴拥军[1,2,3] 赵德刚[1,2] 詹寿年[1] 李耀中[1] 

机构地区：[1]贵州大学生命科学学院,贵州贵阳550025 [2]贵州省农业生物工程重点实验室,贵州贵阳550025 [3]教育部绿色农药与农业生物工程重点实验室,贵州贵阳550025

出　　处：《云南大学学报（自然科学版）》2010年第4期473-479,共7页Journal of Yunnan University(Natural Sciences Edition)

基　　金：国家科技支撑计划资助项目(2007BAD59B06);中国国际合作资助项目(2007DFA31260);国家转基因新品种培育重大专项(2008ZX08010-003)

摘　　要：为探索转ChIFN-γ基因烟草的抗虫作用机制,对虫体蛋白酶活性变化、烟草挥发性成分以及表皮腺毛密度进行了研究.结果表明,烟青虫进食转基因烟草量较少,导致其体内分泌蛋白酶量少、活力低;转基因烟草T0和T1代,黑松三烯和西柏三烯二醇相对含量较对照高;转基因烟草腺毛密度比对照高49.2%.ChIFN-γ基因激活的多种转录因子可能结合了腺毛发育相关基因的cis调节元件,上调了腺毛发育基因的表达,导致腺毛的数量增加,分泌的萜烯化合物增加从而具有显著的抗虫性.To study the insect-resistant mechanism of transgenic ChIFN-γ tobacco,the protease activity of tobacco budworm after feeding on fresh tobacco leaves,tobacco volatile components,and the epidermal trichome density were investigated in the current case.The results showed that insects had a smaller quantity of transgenic tobacco leaves,which may result in less body endocrine protease and lower activity.Compared with the wild type （WT,control）,the T0 and T1 generation of transgenic tobacco demonstrated an obviously higher contents of Duvatriendiol and 4,8,13-Duvatriene-1,3-diol.The density of trichome in transgenic tobacco leaves was 49.2% higher than WT.It possibly happened when some transcription factors were activated by ChIFN-γ and bind the cis-regulatory elements of trichome development-related genes,increased the expression of the trichome-related genes,consequently led to the secretion of terpenoid,which may be ascribed to the main mechanisms of the transgenic ChIFN-γ gene tobaccos.

关 键 词：ChIFN-γ 转基因烟草 腺毛 萜类化合物 抗虫机制 

分 类 号：S572[农业科学—烟草工业]