聚乙烯醇丝固定化米根霉发酵产L(+)-乳酸  

Production of L(+)-Lactic Acid with Immobilized Rhizopus Oryzae As3.3462 in PVA Fibers

作　　者：任慧卿[1] 王远亮[1] 王珍[1] 王润光[1] 高文娟[1] 陈欣[1] 张晓喜[1] 

机构地区：[1]重庆大学生物工程学院生物流变科学与技术教育部重点实验室国家"985工程"生物材料与仿生工程研究中心,重庆400044

出　　处：《精细化工》2010年第7期659-663,666,共6页Fine Chemicals

基　　金：国家自然科学基金项目(No.30870609);重庆市自然科学基金重点项目(No.CSTC2009BA4025)~~

摘　　要：以聚乙烯醇(PVA)丝为吸附材料的立体载体,固定化米根霉菌种As3.3462发酵生产乳酸。与游离发酵相比,摇瓶单批固定化发酵乳酸的最终质量浓度提高了25%,发酵时间缩短了33%。分别在摇瓶和鼓泡式反应器中进行了载体的稳定性研究,摇瓶中可稳定发酵10批次,平均转化率为65%,平均产率为1.1g/(L.h);1L鼓泡式生物反应器的放大实验中进行了9批半连续发酵实验,乳酸质量浓度最高达到55.68g/L,转化率69.60%。A new geometrical immobilization matrix with PVA fibers was introduced to enhance lactic acid production by immobilized Rhizopus oryzae. Compared to the free cell fermentation, final lactic acid increased 25% and fermentation time reduced 33% in shake-flasks. The immobilized-cell fermentation was evaluated for its long-term performance in both of shake-flasks and 1 L bubble-column bioreactor operated in a repeated batch mode. The overall yield of ten cycles in shake-flasks was 65%, corresponding to a volumetric productivity of 1.1 g/（ L · h ）. Moreover, the highest lactic acid concentration of nine cycles in the bubble-column bioreactor was 55.68% with a yield of 69.60% from the consumed glucose.

关 键 词：乳酸 聚乙烯醇丝 固定化 米根霉 生物工程 

分 类 号：TQ921[轻工技术与工程—发酵工程] Q815[生物学—生物工程]