发酵性丝孢酵母发酵产油脂的碳源和氮源选择  

Selection of carbon source and nitrogen source for microbial lipid production by fermentation with Trichosporon fermentans

作　　者：杜凯[1] 孙晓璐 孙玉梅[1] 陈莉[1] 

机构地区：[1]大连工业大学生物与食品工程学院,辽宁大连116034 [2]安徽阜阳一中,安徽阜阳236000

出　　处：《中国油脂》2010年第7期35-38,共4页China Oils and Fats

基　　金：辽宁省教育厅2006年创新团队项目(2006T030)

摘　　要：以发酵性丝孢酵母(Trichosporon fermentans)为实验菌株,通过液体摇瓶发酵研究了碳源和氮源种类对其生长及细胞积累油脂的影响。结果表明:发酵性丝孢酵母以葡萄糖、乳糖、蔗糖及可溶性淀粉作为碳源,以牛肉膏、蛋白胨作为氮源发酵时,菌体生物量和细胞油脂含量均在发酵72 h时达到最大值,继续延长发酵时间不利于油脂积累。以硫酸铵为氮源,木糖为碳源时,菌体最大生长和细胞油脂最大积累不同步。发酵性丝孢酵母发酵产油脂宜选择硫酸铵作为氮源,葡萄糖作为碳源。Various carbon sources and nitrogen sources were used to explore their effects on the growth and microbial lipid accumulation of strain Trichosporon fermentans by submerged fermentation in shaking flask. The results demonstrated that the growth and the lipid accumulation reached maximum at the same time of 72 h, during the fermentation when glucose, sucrose, lactose or soluble amylum was used as a carbon source, and beef extract or peptone was used as a nitrogen source. The growth and the lipid accu- mulation reached maximum at different time during the fermentation when xylose was used as a carbon source and ammonia sulfate was used as a nitrogen source. The eligible nitrogen and carbon source for Trichosporon fermentans to accumulate lipid was ammonia sulfate and glucose.

关 键 词：发酵性丝孢酵母 碳源 氮源 油脂 生长 

分 类 号：TQ642[化学工程—精细化工] TQ92[轻工技术与工程—发酵工程]