溶氧对L-缬氨酸发酵过程的影响  

The Effects of Dissolved Oxygen on L-Valine in Fermentation

作　　者：马雷[1,2] 秦永峰[2] 徐庆阳[2] 谢希贤[2] 

机构地区：[1]天津科技大学电子信息与自动化学院,天津300222 [2]天津科技大学生物工程学院,天津300457

出　　处：《生物技术通讯》2010年第4期509-514,共6页Letters in Biotechnology

基　　金：国家科技重大专项(2008ZX09401-05);天津市应用基础及前沿技术研究计划(08JCZDJC15400);天津市科技支撑计划重点项目(08ZCKFSH01900)

摘　　要：目的:以黄色短杆菌XV0505为供试菌,探索溶氧对L-缬氨酸发酵过程的影响及其控制策略。方法:利用5L发酵罐,考察了不同溶氧浓度对L-缬氨酸发酵的影响,并采用代谢流分析对其结果进行阐述,提出分阶段溶氧控制策略。结果:采用分阶段溶氧控制策略,即在0～24h溶氧浓度为20%,24～60h溶氧浓度为5%,发酵60h,L-缬氨酸可达到58.36g/L,比5%和20%溶氧浓度下分别提高了18.95%和13.56%。结论:溶氧浓度对L-缬氨酸发酵有重要影响。Objective： To explore the effects of dissolved oxygen on the fermentation of L-valine and the strategy for contronling dissolved oxygen with Brevibacterium flavum XV0505 as production strain. Methods： The influence of dissolved oxygen on the L-valine fermentation were stutied. Then the reason was quantitatively described in detail with the distribution of flux for metabolites in metabolic network, and pose that a two-stage oxygen supply strategy. Results： On the optimum oxygen supply strategy, the productivity of L-valine was 58.36 g / L in 5 liter fermentor in 60 h, inceases 18.95% and 13.56% respectively with dissolved oxygen concent rations of 5% and 20%. Conclution： The dissolved oxygen have an important impact on the L-valine production.

关 键 词：L-缬氨酸 溶氧 代谢流分析 发酵 

分 类 号：TQ922[轻工技术与工程—发酵工程]