麦角甾醇酵母基因工程菌株的发酵培养条件  

Fermentation conditions of ergosterol yeast engineering strain

作　　者：宫莉[1] 吴洪发[1] 宋公明[1] 丛娟[1] 李晓玲[1] 裴轶琨[1] 薛冬桦[1] 

机构地区：[1]长春工业大学化学与生命科学学院,吉林长春130012

出　　处：《吉林大学学报（医学版）》2010年第4期807-810,共4页Journal of Jilin University：Medicine Edition

基　　金：吉林省教育厅重点项目资助课题(200677);吉林省长春市科技局国际合作项目资助课题(2006233)

摘　　要：目的:探讨麦角甾醇酵母基因工程菌株的发酵培养条件,阐明重组菌株可以玉米水解液为发酵培养基代谢合成麦角甾醇。方法:采用不同的培养基质、氮源、玉米浆含量、无机盐、pH检测工程菌株菌株细胞生物量和麦角甾醇含量。结果:发酵工艺参数为玉米水解液10oBx、玉米浆6.5%、硫酸镁0.1%,pH值6.5,接种量10%,28℃摇床振荡培养16h,酵母工程菌(YDH/pGAD-ScERG1)细胞生物量达14.34g.L-1,麦角甾醇含量可达3.05%,麦角甾醇得率为435.93mg·mL-1。结论:酵母工程菌株(YDH/pGAD-ScERG1)ERG1基因的表达有利麦角甾醇合成。Objective To explore the fermentation conditions of ergosterol yeast engineering strain and illustrate that the yeast strain（YDH/pGAD-ScERGI）can synthesize ergosterol by using corn hydrolysates as fermentation substrates.Methods The different substrates,nitrogen sources,corn syrup contents,inorganic salts,pH were used to measured the cell biomass of engineered strain and ergosterol content.Results When the fermentation parameters of corn hydrolysates 10o Bx,corn steep 6.5%,magnesium sulfate 0.1%,initial pH of 6.5,inoculum 10%,28℃ shaking cultured 16 h,the yeast（YDH/pGAD-ScERG1）cell biomass reached 14.34 g·L-1,the ergosterol content was up to 3.05%,ergosterol yield was 435.93 mg·L-1.Conclusion The ERG1 gene expression of yeast strains（YDH/pGAD-ScERG1）is conducive to the synthesis of ergosterol.

关 键 词：麦角甾醇 酵母菌株 发酵 玉米水解液 玉米浆 

分 类 号：R344.83[医药卫生—基础医学]