磁性壳聚糖微球固定化β-呋喃果糖苷酶的研究  

Study on β-fructofuranosidase immobilized with magnetic chitosan microspheres

作　　者：许牡丹[1] 曾令军[1] 姚甲玉[1] 刘红梅[1] 

机构地区：[1]陕西科技大学生命科学与工程学院,陕西西安710021

出　　处：《食品工业科技》2010年第8期180-182,共3页Science and Technology of Food Industry

摘　　要：以磁性壳聚糖微球为载体,采用戊二醛交联法固定化β-呋喃果糖苷酶,并对固定化酶的理化性质等进行了研究。结果表明:固定化β-呋喃果糖苷酶与游离酶相比,具有较强的耐碱性,在温度低于70℃范围内有良好的热稳定性;固定化酶在4℃下保存15d后,剩余酶活力为80%左右,具有良好的储存稳定性,连续10次操作使用后保持50%的酶活,具有良好的操作性,固定化酶回收率为26%。β-fructofuranosidase was immobilized by cross linking with glutaraldehyde onto magnetic polyvinylalcohol microsphere.And the physical and chemical properties of the immobilized enzyme were studied. The results showed that the immobilized β-fructofuranosidase had stronger resistance to alkali compared with the free enzyme.In less than 70℃ situation,the immobilized β-fructofuranosidase showed good thermal stability.The recovery activity was 80% when the immobilized β-fructofuranosidase was stored at 4℃ for 15d.The recovery activity of the immobilized β-fructofuranosidase was more than 50% after repeated use for 10 times and immobilized enzyme recovery was 26%.

关 键 词：磁性壳聚糖微球 Β-呋喃果糖苷酶 固定化酶 

分 类 号：TS201.25[轻工技术与工程—食品科学]