Sevoflurane postconditioning reduces myocardial reperfusion injury in rat isolated hearts via activation of PI3K/Akt signaling and modulation of Bcl-2 family proteins  

作　　者：Li-na YU Jing YU Feng-jiang ZHANG Mei-juan YANG Ting-ting DING Jun-kuan WANG Wei HE Tao FANG Gang CHEN Min YAN 

机构地区：[1]Department of Anesthesiology, the Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China [2]Department of Anesthesiology, Jinhua People's Hospital Jinhua 321300, China

出　　处：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》2010年第9期661-672,共12页浙江大学学报（英文版）B辑（生物医学与生物技术）

基　　金：supported by the National Natural Science Foundation of China (No. 30772090);the Natural Science Foundation of Zhejiang Province (Nos. Y204141 and R2090259);the Foundation from Science and Technology Department of Zhejiang Province (No. 2007R10034);the Foundation from the Health Bureau of Zhejiang Province (No. 2007QN007),China

摘　　要：Sevoflurane postconditioning reduces myocardial infarct size.The objective of this study was to examine the role of the phosphatidylinositol-3-kinase(PI3K)/Akt pathway in anesthetic postconditioning and to determine whether PI3K/Akt signaling modulates the expression of pro-and antiapoptotic proteins in sevoflurane postconditioning.Isolated and perfused rat hearts were prepared first,and then randomly assigned to the following groups:Sham-operation(Sham),ischemia/reperfusion(Con),sevoflurane postconditioning(SPC),Sham plus 100 nmol/L wortmannin(Sham+Wort),Con+Wort,SPC+Wort,and Con+dimethylsulphoxide(DMSO).Sevoflurane postconditioning was induced by administration of sevoflurane(2.5%,v/v) for 10 min from the onset of reperfusion.Left ventricular developed pressure(LVDP),left ventricular end-diastolic pressure(LVEDP),maximum increase in rate of LVDP(+dP/dt),maximum decrease in rate of LVDP(?dP/dt),heart rate(HR),and coronary flow(CF) were measured at baseline,R30 min(30 min of reperfusion),R60 min,R90 min,and R120 min.Creatine kinase(CK) and lactate dehydrogenase(LDH) were measured after 5 min and 10 min reperfusion.Infarct size was determined by triphenyltetrazolium chloride staining at the end of reperfusion.Total Akt and phosphorylated Akt(phospho-Akt),Bax,Bcl-2,Bad,and phospho-Bad were determined by Western blot analysis.Analysis of variance(ANOVA) and Student-Newman-Keuls' test were used to investigate the significance of differences between groups.The LVDP,±dP/dt,and CF were higher and LVEDP was lower in the SPC group than in the Con group at all points of reperfusion(P<0.05).The SPC group had significantly reduced CK and LDH release and decreased infarct size compared with the Con group [(22.9±8)% vs.(42.4±9.4)%,respectively;P<0.05].The SPC group also had increased the expression of phospho-Akt,Bcl-2,and phospho-Bad,and decreased the expression of Bax.Wortmannin abolished the cardioprotection of sevoflurane postconditioning.Sevoflurane postconditioning may protect the isolated rat heart.Activation of PI3KSevoflurane postconditioning reduces myocardial infarct size.The objective of this study was to examine the role of the phosphatidylinositol-3-kinase（PI3K）/Akt pathway in anesthetic postconditioning and to determine whether PI3K/Akt signaling modulates the expression of pro-and antiapoptotic proteins in sevoflurane postconditioning.Isolated and perfused rat hearts were prepared first,and then randomly assigned to the following groups：Sham-operation（Sham）,ischemia/reperfusion（Con）,sevoflurane postconditioning（SPC）,Sham plus 100 nmol/L wortmannin（Sham＋Wort）,Con＋Wort,SPC＋Wort,and Con＋dimethylsulphoxide（DMSO）.Sevoflurane postconditioning was induced by administration of sevoflurane（2.5%,v/v） for 10 min from the onset of reperfusion.Left ventricular developed pressure（LVDP）,left ventricular end-diastolic pressure（LVEDP）,maximum increase in rate of LVDP（＋dP/dt）,maximum decrease in rate of LVDP（？dP/dt）,heart rate（HR）,and coronary flow（CF） were measured at baseline,R30 min（30 min of reperfusion）,R60 min,R90 min,and R120 min.Creatine kinase（CK） and lactate dehydrogenase（LDH） were measured after 5 min and 10 min reperfusion.Infarct size was determined by triphenyltetrazolium chloride staining at the end of reperfusion.Total Akt and phosphorylated Akt（phospho-Akt）,Bax,Bcl-2,Bad,and phospho-Bad were determined by Western blot analysis.Analysis of variance（ANOVA） and Student-Newman-Keuls＇ test were used to investigate the significance of differences between groups.The LVDP,±dP/dt,and CF were higher and LVEDP was lower in the SPC group than in the Con group at all points of reperfusion（P0.05）.The SPC group had significantly reduced CK and LDH release and decreased infarct size compared with the Con group [（22.9±8）% vs.（42.4±9.4）%,respectively;P0.05].The SPC group also had increased the expression of phospho-Akt,Bcl-2,and phospho-Bad,and decreased the expression of Bax.Wortmannin abolished the cardioprotection of sevoflurane p

关 键 词：SEVOFLURANE POSTCONDITIONING CARDIOPROTECTION Akt Bcl-2 BAD 

分 类 号：R614[医药卫生—麻醉学]