反相高效液相色谱法检测食品添加剂乳铁蛋白  

Study of Determination of Food Additive Lactoferrin by High Performance Liquid Chromatography

作　　者：张林田[1] 陈小雪[1] 魏建华[1] 张冬辉[1] 陈建伟[1] 

机构地区：[1]汕头出入境检验检疫局

出　　处：《食品研究与开发》2010年第8期130-132,共3页Food Research and Development

基　　金：国家质检总局项目(20071903-T-424)

摘　　要：建立反相高效液相色谱法检测食品添加剂乳铁蛋白的方法。样品采用氯化钠溶液（0.5mol/L）溶解,过滤后供液相色谱仪测定。采用CAPCELL SG300 C18色谱柱分离,氯化钠溶液（0.5mol/L）、乙腈及三氟乙酸为流动相,梯度洗脱,二极管阵列检测器280nm检测,保留时间及特征光谱图定性,外标法峰面积定量测定。标准曲线线性范围10.0mg/L～100mg/L,相关系数均在0.999以上,定量检测低限5mg/kg。A determination of food additive lactoferrin by reverse phase high-performance liquid chromatography is described.The lactoferrin is extracted from the samples with the sodium chloride solution of 0.5mol/L,filtered,and analyzed at 280 nm by high-performance liquid chromatography with DAD.The chromatographic separation is carried out on CAPCELL SG300 C18 column with ramp mobile phase using the sodium chloride solution of 0.5mol/L,acetonitrile and trifluoroacetic acid.The qualitation of lactoferrin depends on the RT and characteristic UV spectra;quantified by external standard calibration curves.The calibration curve is linear in the 10.0mg/L-100mg/L range with correlation coefficients≥0.999.The limit of detection and quantification is 5mg/kg.The analytical method is simple,fast and precise with a good sensitivity,an excellent reproducibility.

关 键 词：高效液相色谱 添加剂 乳铁蛋白 

分 类 号：TS207.3[轻工技术与工程—食品科学]