环介导等温扩增技术检测肉及肉制品中的沙门氏菌  

Loop-mediated Isothermal Amplification Assay for the Detection of Salmonella in Meat and Meat Products

作　　者：王羽[1] 王贞强[1] 张伟[1] 

机构地区：[1]河北农业大学食品科技学院,河北保定071000

出　　处：《食品科学》2010年第16期270-273,共4页Food Science

基　　金：河北省自然科学基金项目(C2008000216)

摘　　要：建立用环介导等温扩增技术(loop-mediated isothermal amplification,LAMP)检测肉及肉制品中沙门氏菌的方法。利用靶基因的6个区段设计4条引物,应用具有链置换活性的Bst DNA聚合酶,能够在恒温(65℃左右)条件下特异、高效、快速地扩增待测物的DNA。针对沙门氏菌的特异基因invA基因设计两对LAMP引物,建立LAMP检测沙门氏菌的新方法,并对肉及肉制品中的沙门氏菌进行检测。结果表明:建立LAMP技术检测肉及肉制品中沙门氏菌的方法,该方法能够特异检测沙门氏菌,且灵敏性可达10-9CFU/mL,对48份样品检测,该方法检出率为91.6%、敏感性100%、特异性70.0%、符合率为93.8%、检出时间1.5h。A novel nucleic acid amplification method, termed loop-mediated isothermal amplification （LAMP）, which amplifies DNA with high specificity, efficiency, and rapidity under isothermal conditions, may be a valuable tool for the rapid detection of infectious diseases. In this study, two pairs of gene-specific primers for the conservative invA gene were designed to establish a LAMP assay for detecting Salmonella. The assay method was applied to detect meat of different animal species, and proved highly sensitive and specific and time saving.

关 键 词：环介导等温扩增 检测 沙门氏菌 肉及肉制品 

分 类 号：Q939.1[生物学—微生物学] Q789