多优良性状工业化酿酒酵母的选育及其特性研究  

Screening of mult-traits industrial strain of Saccharomyces cerevisiae and its characters

作　　者：关妮[1] 杨登峰[1] 米慧芝[1] 陆琦[1] 黄日波[1] 

机构地区：[1]广西科学院国家非粮生物质能源工程技术研究中心,广西南宁530007

出　　处：《中国酿造》2010年第9期45-48,共4页China Brewing

基　　金：国际科技合作项目(2009DFA30820);广西科技攻关项目(桂科自0991077);广西科学院基金项目(YJ16SW03);(10YJ25SW14)

摘　　要：通过双亲灭活的原生质体融合技术,将高效的酿酒酵母gxas02和絮凝酿酒酵母A3融合育种,得到的融合子R2-4能在短时间内产生高浓度酒精(发酵液中酒精含量为16.20%vol),发酵时间至少缩短8h,残糖0.076g/L,糖的利用接近理论值,耐酒精能力提高,有一定的工业应用潜力。A fusion Saccharomyces cerevisiae strain R2-4, which could produce ethanol at high gravity （16.20%vol ethanol in fermentation broth） in short time, was constructed by protoplast fusion of heat-inactivated S. cerevisiae gxas02 and UV-inactivated flocculating S. cerevisiae A3. The fermentation time was shortened at least 8h with strain R2-4. Besides, the utilization of glucose was very close to the theoretical value and the residual sugar was only 0.076g/L at the end of fermentation. The ethanol tolerance capacity of S. cerevisiae was improved by protoplast fusion. And the strain R2-4 was a good candidate for industrial application.

关 键 词：原生质体融合 酿酒酵母 燃料乙醇 

分 类 号：TS261.1[轻工技术与工程—发酵工程]