藏灵菇酵母菌K1耐胃肠道逆环境特性及降胆固醇的实验研究  

Tolerance characteristics of the gastrointestinal tract and cholesterol-lowering effects of Kluyveromyces marxianus K1 from Tibetan Kefir

作　　者：刘慧[1] 李晨[1] 杨磊[1] 贾慧[1] 张红星[1] 

机构地区：[1]北京农学院食品科学学院,北京102206

出　　处：《中国酿造》2010年第9期61-64,共4页China Brewing

基　　金：北京市自然科学基金资助项目(No.5092008);北京市重点建设学科资助项目(PXM2009_014207_078172)

摘　　要：利用从藏灵菇中筛选的马克斯克鲁维酵母K1菌株探讨胃肠道耐受性及体外降胆固醇功效。分别在pH 1.5~4.5、胆盐浓度0.3%~3.0%、NaCl浓度3.0%~6.0%的模拟胃肠道环境下,采用活菌计数法检测K1菌株的耐胃肠道逆环境特性,以邻本二甲醛法检测其降胆固醇效果。结果表明,在pH2.5~4.5作用4h活菌数超过106cfu/mL,0.3%~3.0%胆盐及3.0%~6.0%NaCl作用8h活菌数为104cfu/mL^106cfu/mL,在pH1.5~3.0(4h)及胆盐浓度0.3%~3.0%的模拟人体胃肠道环境下降胆固醇率40.0%~52.8%。显示K1菌株具有较强的耐胃肠道逆环境特性及良好的降胆固醇能力。Tolerance characteristics of gastrointestinal tract and/n vitro cholesterol-lowering effects of Kluyveromyces marxianus K1 isolated from Tibetan Kefir were studied. Tolerance characteristics of the gastrointestinal tract of strain K1 were studied using viable count with pH value 1.5--4.5, bile salt concentration 0.3%-3.0%, sodium chloride 3.0%---6.0%, which simulated gastrointestinal tract environment. Effect of cholesterol-lowering was evaluated with O-phthalaldehyde. The results showed that viable count was 〉106 cfu/ml under pH value 2.5---4.5 after 4 h, and it was in the range of 104-106 cfu/ml when bile salt was in the range of 0.3%---3.0%, concentration of sodium chloride was 3.0%---6.0% after 8 h. Rate of cholesterol-low- ering can reach 40.0%-52.8% under simulated gastrointestinal tract environment, which were pH value 1.5-3.0 treated for 4 h and salt bile concentration 0.3%-3.0% treated for 8 h. The results indicated that K1 strain has strong tolerance against environment of the gastrointestinal tract and good capacity of cholesterol-lowering, which provided basis for development of cholesterol-lowering functional foods and probiotics.

关 键 词：马克斯克鲁维酵母 耐受性 胃肠道环境 降胆固醇 

分 类 号：Q93-331[生物学—微生物学]