大肠杆菌O157的志贺毒素基因克隆和测序  

Gene cloning and sequence analysis of Shiga toxin-producing Escherichia coli O157

作　　者：李睿[1] 孙言凤[1] 王芳[2] 熊燕[2] 

机构地区：[1]武汉工业学院生物与制药工程学院,湖北武汉430023 [2]武汉市疾病预防控制中心,湖北武汉430015

出　　处：《中国酿造》2010年第9期65-67,共3页China Brewing

基　　金：2009湖北省自然科学基金重点课题(2009CDA118)

摘　　要：将一株大肠杆菌O157PCR扩增stx2基因全长并克隆测序。该菌株stx2基因与GenBank数据库收录的stx2基因最高同源性为98%,在3个核苷酸位点存在基因突变。采用邻位相连法构建进化树,序列分析结果表明O157为stx2C基因亚型。了解大肠杆菌O157的基因突变情况,并为开发大肠杆菌分子检测方法提供了参考。The full length of stx2 genes from an E. coli O 157 strain was amplified by PCR assay, cloned into vector and sequenced. There were three varied nucleotide sites of stx2 genes of the O157 strain, and they were 98% similarity with stx2 sequences deposited in GenBank database. The phylogenetic tree was constructed by neighbor-joining method and sequence analysis demonstrated that stx2 genes of the O 157 strain belonged to stx2c genotype. This study would be helpful for understanding of gene mutation of O157 isolate, and provided reference for development of molecular detection methods for E. coll.

关 键 词：大肠杆菌O157 志贺毒素基因 基因克隆 测序 

分 类 号：R155.5[医药卫生—营养与食品卫生学]