稀土元素对2-酮基-L-古龙酸发酵过程的影响  

Effect of Rare Earth Elements on Fermentation Process of 2-Keto-L-gulonic Acid

作　　者：郑巧双[1,2] 周景文[1,2] 陈孚江[1,2] 刘杰[3] 刘立明[1,2] 陈坚[1,2] 

机构地区：[1]江南大学食品科学与技术国家重点实验室,江苏无锡214122 [2]江南大学工业生物技术教育部重点实验室,江苏无锡214122 [3]江苏江山制药有限公司,江苏靖江214500

出　　处：《过程工程学报》2010年第4期750-755,共6页The Chinese Journal of Process Engineering

基　　金：国家杰出青年基金资助项目(编号:20625619);国家高技术研究发展计划(863)基金资助项目(编号:2006AA020303);国家十一五科技支撑计划基金资助项目(编号:2007BAI46B02);国家重点基础研究发展规划(973)基金资助项目(编号:2007CB714303)

摘　　要：采用普通生酮基古龙酸菌(Ketogulonigenium vulgare)与巨大芽孢杆菌(Bacillus megaterium)混合菌系,研究了不同稀土离子及其配合物对发酵生产维生素C前体物质2-酮基-L-古龙酸(2-KLG)的影响.结果表明,各稀土离子对2-KLG合成的最佳促进浓度均约为5mmol/L,且具有低浓度促进、高浓度抑制的作用规律.EDTA镧、柠檬酸镧均抑制2-KLG的合成,而1mmol/L草酸镧对其合成代谢有促进作用,使其产量提高6.4%.摇瓶中添加5mmol/LLa^(3+),发酵中后期菌体生长状况得到显著改善的同时提高了2-KLG最终产量并缩短了发酵周期.3.6L发酵罐中添加5mmol/LLa^(3+)进行分批发酵,平均山梨糖消耗速率和2-KLG生产强度比对照分别提高12.4%和14.8%,发酵周期缩短11.1%.The effects of different rare earth ions and coordination compounds on the synthesis of 2-keto-L-gulonic acid（2-KLG） by mixed culture of Bacillus megaterium and Ketogulonigenium vulgare were investigated in the two-step fermentation of vitamin C.The results indicated that the proper promotion concentrations of various rare earth ions were all about 5 mmol/L.Furthermore,the low concentration caused 2-KLG synthesis promotion while high concentration caused 2-KLG synthesis inhibition.EDTA-La and lanthanum citrate inhibited 2-KLG synthesis,but lanthanum oxalate promoted it at a concentration of 1 mmol/L and enhanced the final production of 2-KLG by 6.4%.The cell growth conditions at the mid-and late fermentation stages were remarkably improved with the addition of 5 mmol/L La^（3＋）.2-KLG ultimate output was increased and the fermentation time reduced at the same time.When 5 mmol/L La^（3＋） was fed to 3.6 L fermentor for batch fermentation,the L-sorbose consumption rate and 2-KLG productivity were increased by 12.4% and 14.8% compared with that of the control,respectively.And the fermentation time was declined by 11.1%.

关 键 词：二步发酵 稀土元素 2-酮基-L-古龙酸 稀土配合物 

分 类 号：Q939.9[生物学—微生物学]