鳕鱼多肽的抗氧化活性及其分离纯化  

Antioxidant Activity and Purification of Pollock Peptides

作　　者：牛瑞[1] 于建生[1] 

机构地区：[1]青岛科技大学化工学院,青岛266042

出　　处：《食品与生物技术学报》2010年第4期562-566,共5页Journal of Food Science and Biotechnology

摘　　要：研究了鳕鱼多肽的抗氧化活性及分离纯化。为获得高纯度的抗氧化肽,将鳕鱼多肽依次通过超滤、凝胶过滤层析、阴离子交换层析(Q-FF)和反相高效液相色谱层析(RP-HPLC)进行分离纯化。结果表明:鳕鱼多肽具有很强的清除羟自由基的能力、清除DPPH的能力和还原能力;经Q-FF柱层析分离得到了B1、B2和B33个组分,B2经RP-HPLC检测显示为单一峰,获得了纯度较高的鳕鱼多肽,这为鳕鱼多肽的开发利用提供了科学理论依据。The antioxidant activities of Pollock peptides were studied at first in this manuscript and demonstrated he Pollock peptides had strong scavenging hydroxyl free radical activity,and scavenging 1,diphenyl-2-picrylhydrazyl（DPPH） activity,and reducing activity.In order to obtain high homogenous antipeptide,the Pollock peptides were purified by ultraffltrations,gel filtration,anion-exchange chromatography（Q-FF）,and reversed phase high performance liquid chromatography（RP-HPLC） in turn.These results showed that Pollock peptides separated on Q-FF column and three components（B1,B2,B3） were obtained.B2 was identified by RP-HPLC to be homogenous.

关 键 词：鳕鱼多肽 抗氧化活性 分离纯化 

分 类 号：R151.2[医药卫生—营养与食品卫生学]