黏度指标考察哈蟆油酶解工艺的研究  

Study on the enzyme hydrolysis technology of Oviductus ranae with viscosity as the index

作　　者：刘诗超[1] 赵雨[2] 张梅[2] 李芸彤[2] 金光洙[1] 

机构地区：[1]延边大学药学院,延吉133000 [2]长春中医药大学中医药与生物工程研发中心,长春130117

出　　处：《食品科技》2010年第9期133-135,139,共4页Food Science and Technology

基　　金：国家"十一五"科技支撑项目(2007BAI38B06)

摘　　要：目的:确定哈蟆油最佳酶解工艺。方法:以黏度为指标,通过单因素试验及L9(33)正交试验,考察酶用量、酶解温度、酶解时间对哈蟆油黏度的影响,确定哈蟆油最佳酶解工艺。结果:哈蟆油最佳酶解条件为:酶用量120 U/g,酶解温度65℃,酶解时间40 min。结论:酶解后的哈蟆油由最初黏度3127 mPa.s降为8.86 mPa.s,具有更好的流动性及溶解性。该酶解工艺方法简单、稳定、可行。Objective：To establish the optimal enzymatic hydrolysis technology of Oviductus ranae.Methods： The monofactor test and the L9（33） orthogonal test are used to select the best technique condition with viscosity as the index,while the enzyme dosage,the enzymolysis temperature and the enzymolysis time as the observation factors.Results： The optimum enzymatic hydrolysis technology is obtained as follows： the enzyme dosage is 120 U/g,the enzymolysis temperature is 65 ℃ and the enzymolysis time is 40 min.Conclusion： The viscosity of Oviductus Ranae reduces from 3127 mPa.s to 8.86 mPa.s and the production dissolubility and flowability are both better.The enzymatic hydrolysis technology is simple,stable and feasible.

关 键 词：哈蟆油 黏度 酶解工艺 正交试验 

分 类 号：R284.2[医药卫生—中药学]