双抗体夹心ELISA定量检测牛乳中酪蛋白方法的建立  

Development of sandwich-antibody enzyme linked immunosorbent assay for the rapid detection of casein in milk

作　　者：王硕[1] 杨泽琳[1] 马冬月[1] 段素欣[1] 张燕[1] 李细芬[1] 江月明[1] 房立[1] 

机构地区：[1]天津科技大学,天津300457

出　　处：《食品科技》2010年第9期290-293,共4页Food Science and Technology

基　　金：国家"十一五"科技支撑计划项目(2009BADB9B03)

摘　　要：实验使用酪蛋白标品为抗原,免疫新西兰大白兔制备抗酪蛋白的多克隆抗体,并免疫BALB/C小鼠制备抗酪蛋白单克隆抗体,以单克隆抗体包被,HRP标记多抗,成功地建立了检测牛乳中酪蛋白的双抗体夹心ELISA法,标准曲线在6～1666 ng/mL范围内线性良好,r2=0.9949,最低检出限为7 ng/mL,具有很好的特异性。通过建立双抗体夹心ELISA法测定酪蛋白含量,可为牛乳掺假和食物过敏原的检测及标签管理提供理论及技术基础。Casein was used as the antigen to immune the New Zealand rabbits and BALB/C mice respectively in the experiment.The anti-casein polyclonal antibodies and monoclonal antibodies were prepared.The monoclonal antibody was used as the coating antibody,and HRP-polyclonal antibody was retained.The sandwich ELISA to detect the casein of milk was developed successfully,which had a standard curve in the 6～ 1666 ng/mL range of good linearity with r2=0.9949.The detection limit of the method was 7 ng/mL.The method could be use as a theory and technological base of casein detection of milk adulteration and food allergen test.

关 键 词：夹心ELISA 多克隆抗体 单克隆抗体 酪蛋白 

分 类 号：TS207.3[轻工技术与工程—食品科学]