南瓜多糖大孔吸附树脂纯化工艺  

Purification Technology of Pumpkin Polysaccharides with Macroporous Adsorption Resin

作　　者：王丽波[1] 赵玉[1] 徐雅琴[1] 

机构地区：[1]东北农业大学理学院,哈尔滨150030

出　　处：《农业机械学报》2010年第9期138-142,共5页Transactions of the Chinese Society for Agricultural Machinery

基　　金：黑龙江省自然科学基金资助重点项目(ZJN0606-01)

摘　　要：为提高南瓜多糖的纯度,筛选适宜南瓜多糖纯化的大孔树脂,确定纯化工艺参数,选用9种大孔树脂材料,采用静态与动态吸附-解吸方法对南瓜多糖进行纯化,以吸附量与解吸率为考察指标对树脂进行选择与工艺研究。结果表明:D101-Ⅰ型大孔树脂更适宜作为南瓜多糖纯化的树脂;其纯化最佳工艺条件为:上柱液质量浓度4.79 mg/mL,上柱液体积3.2 BV,上柱流速3 BV/h,洗脱液乙醇体积分数25%,洗脱速度4 BV/h,洗脱液体积3.6 BV;上述纯化工艺使南瓜多糖纯度从25.68%提高到61.39%。In order to select suitable macroporous resin,to improve the purity of pumpkin polysaccharide and to determine purification process parameters,9 kinds of macroporous resin were chosen to carry out static and dynamic adsorption-desorption experiments.With the indexes of adsorption capacity and elution rate,the suitable macroporous resin was selected and process parameters were confirmed.The results show that D101-I resin has the best performance of adsorption and desorption effects for purification of pumpkin polysaccharide,the optimum technology parameters are sample concentration of 4.79 mg/mL,sample volume of 3.2 BV,flow rate of 3 BV/h,25% ethanol for elution,elution velocity of 4 BV/h,elution volume of 3.6 BV.The purity of pumpkin polysaccharide increases from 25.68% to 61.39% by using the above technology.

关 键 词：南瓜多糖 大孔吸附树脂 分离纯化 

分 类 号：TS244[轻工技术与工程—制糖工程]