响应面法优化橙皮苷酶法改性工艺研究  

Optimization of Process Technology for Enzymatic Modification of Hesperidin by Response Surface Methodology

作　　者：盛占武[1,2] 金志强[1,2] 高锦合[1] 马蔚红[1] 孙志高[3] 

机构地区：[1]中国热带农业科学院海口实验站,海口570102 [2]中国热带农业科学院热带生物技术研究所,海口571101 [3]中国农业科学院柑桔研究所,重庆400712

出　　处：《中国食品学报》2010年第4期202-210,共9页Journal of Chinese Institute Of Food Science and Technology

基　　金：海南省自然科学基金项目(309043);中央级科研院所基本科研业务费重点项目(ITBBKF2008-2)

摘　　要：以橙皮苷为原料,利用响应面法优化环糊精葡萄糖基转移酶改性橙皮苷的工艺条件。根据单因素方差分析结果确定影响橙皮苷转化率的主要因素是:反应体系pH、温度和加酶量;利用响应面回归分析方法对反应条件进行优化,得出最佳反应条件是:温度55℃、pH7、加酶量800U/g;通过验证试验得出转化率为78.03%,与模型预测数值(79.79%)没有明显的差异,可信度较高;在最佳反应条件下橙皮苷转化率为79.79%,比已报道(70%)的高。The study investigated the process technology of enzymatic modification of hesperidin,using cyclodextrin glucanotransferase（CGTase）.The ranges of treatment variables of CGTase concentration,pH and temperature on transformation rate of hepseridin were confirmed through single factor experiment.Response surface methodology（RSM） was conducted to get the optimal parameters.The results indicated that transformation rate of hepseridin was mainly affected by pH,CGTase concentration and temperature.The generated regression model represented the relationship between the independent variables and the responses.From the RSM-generated model,the optimum conditions of enzymatic treatment were identified as CGTase concentration 800 U/g,pH 7 and temperature 55 ℃.Under the optimum conditions,the predicted transformation rate of hepseridin was 79.79%,whereas,the experimental transformation rate of hepseridin was 78.03%.The RSM-predicted and experimental values were not significantly different from each other.The enzymatic modification of hesperidin under the optimum reaction conditions obtained significantly more modified hesperidin（79.79%） than prior reported method（70%）.

关 键 词：橙皮苷 环糊精葡萄糖基转移酶 酶法改性 响应面 优化 

分 类 号：TQ464.3[化学工程—制药化工]