22种黄酮、酚酸类化合物和9种中药清除AAPH活性考察  

AAPH scavenging activities of 22 flavonoids and phenolic acids and 9 extracts of Chinese materia medica

作　　者：戴卉卿[1,2] 陈承瑜[1] 杨滨[1] 

机构地区：[1]中国中医科学院中药研究所,北京100700 [2]江西中医学院,江西南昌330004

出　　处：《中国中药杂志》2010年第17期2296-2302,共7页China Journal of Chinese Materia Medica

基　　金：国家"重大新药创新"科技重大专项(2009ZX09308-005);国际科技合作项目(2006DFB31720)

摘　　要：目的:考察中药中黄酮、酚酸类成分清除2,2′-azobis(2-methylpropionamidine)dihydrochloride(AAPH)的活性及构效关系,中药提取物清除AAPH的活性与其酚类成分之间的相关性。方法:以中药中常见的22种黄酮、酚酸类化合物和9种中药为研究对象,以Oxygen Radical Absorbance Capacity(ORAC)法对上述31个样品清除AAPH自由基的活性进行评估,以Folin-Ciocalteu法测定中药中黄酮和酚酸类成分的总量,以高效液相色谱-紫外检测技术对中药的抗氧化活性成分进行定性定量检测。结果:茶叶清除AAPH自由基能力最强,ORAC值为4 786.40μmol.g-1;红花醇提物最弱,ORAC值784.04μmol.g-1。有效成分中,槲皮素清除AAPH自由基能力最强,ORAC值为12.90μmol.g-1;(-)-EGC最弱,ORAC值2.47μmol.g-1。中药清除AAPH自由基能力(Y)与总黄酮和酚酸类成分含量(X)之间存在相关性,Y=1 844.8Ln(X)-3 577.5,r=0.867 5。结论:9种中药清除AAPH自由基的有效成分为黄酮和酚酸类成分,这些化合物清除AAPH自由基的能力与其立体构型、是否糖苷化及酯化等因素有关。将ORAC法与高效液相色谱法相结合,对中药抗氧化活性及有效成分进行研究,是一个切实可行的方法。Objective： To investigate the AAPH scavenging activities of 22 flavonoids and phenolic acids and 9 extracts of Chinese materia medica.Method： The antioxidant activities of the samples were evaluated by an oxygen radical absorbance capacity method（ORAC）,at the same time,the total contents of flavonoids and phenolic the 9 herb extracts were analyzed by Folin-Ciocalteu method,and the active components were qualitatively and quantitatively analyzed by an HPLC method.Result： It was found that the tea extract showed the strongest AAPH activity with the ORAC value of 4 786.40 μmol·g-1 whereas safflower demonstrated the weakest activity with the ORAC value of 784.04 μmol·g-1.As for compounds,quercetin had the strongest AAPH activity with the ORAC value of 12.90 while（-）-EGC had the weakest activity with the ORAC value of 2.47.A quantitative relationship was obtained to describe the AAPH scavenging activity of the herb extracts： Y=1 844.8lnX-3 577.5,r=0.867 5,where Y stands for the ORAC vaule,and X stands for the concentration of total phenolic acids.Conclusion： Flavonoids and phenolic acids are the AAPH scavenging active ingredients in the Chinese herb extracts.It′s a good way to study the antioxidant activity of Chinese herb extract and its chemical composition by combing ORAC method and HPLC method.

关 键 词：中药 AAPH ORAC 酚类成分 定性定量分析 

分 类 号：R284[医药卫生—中药学]