赭绿青霉Sp1菌株木聚糖酶最佳产酶条件及部分酶学特性研究  

Fermentation and Characteristics of A Xylanase Producing Strain Penicillium ochro-chloron Sp1

作　　者：冯蕾[1,2,3] 宋彦波[1,2] 徐晓立[1,2] 罗思施[1,2] 杨新平[3] 陈竞[3] 秦新政[3] 唐娴[3] 谢君[1,2] 

机构地区：[1]华南农业大学应用生物化学研究所,广州510642 [2]广东省高校生物质能源重点实验室,广州510642 [3]新疆农业科学院微生物研究所,乌鲁木齐830091

出　　处：《新疆农业科学》2010年第8期1606-1614,共9页Xinjiang Agricultural Sciences

基　　金：广东省;国家科技部产学研合作专项(2007B090400054);2008年广州市科技攻关项目(2008Z1-B341);2009年广东省中国科学院全面战略合作项目(2009B091300098);新疆维吾尔自治区科技攻关项目(200931102);新疆维吾尔自治区高技术发展计划项目(201010104)

摘　　要：【目的】从土壤中分离筛选获得了一株具有高产木聚糖酶能力的真菌Sp1。【方法】经过28SrDNA测序鉴定为赭绿青霉(Penicillium ochro-chloron)。通过对摇瓶发酵条件优化试验及酶特性研究。【结果】该菌株的最适产酶条件为:2%(W/V)粗制木聚糖+0.5%葡萄糖为碳源,0.44%KNO3+0.06%蛋白胨为氮源,发酵起始pH为5.0,150mL三角瓶装液量为40mL,转速为220r/min,28℃下培养72h木聚糖酶酶活可达387U/mL。将菌株Sp1发酵所得粗酶液经过纯化后研究其酶学特性,酶最适反应pH为4.0,最适反应温度为55℃,在40℃,pH2.2～6.0,酶活性稳定。终浓度为0.01mol/L的Na+、K+和Zn2+对酶活有促进作用。【结论】所筛选的Sp1菌株具有较好的应用前景。[Objective and Method]The aim of this study was designed to screen a strain of fungi named Sp1 from the soil.It was identified as Penicillium ochro-chloron by morphological identification and 28S rDNA sequence analysis.[Result]The results showed that the optimum conditions for Sp1 producing xylanase were mixture of 2%（W/V）xylan,0.5%glucose,mixture of 0.44%KNO3 and 0.06% peptone,40 mL liquid medium in 150 mL flask which is composed of 0.2%KH2PO4,0.2%（NH4）2SO4,0.03% MgSO4·7H2O,0.03% CaCl2,0.0005 %FeSO4·7H2O,2%xylan,0.5%glucose,0.44% KNO3,the initial pH 5.0,fermentable temperature 28 ℃,200 rpm of rotation,fermentation time was 3 days,the enzyme activity of xylanase reached 387 U/mL.the crude enzyme from SP1 strain was separated and purified and indicated that the purified xylanase was endo-xylanase.The optimal pH and temperature were 4.0 and 55℃,good stability below 55℃,stable within pH 2.5～5.5.Na^2＋,K^＋ and Zn^2＋ can activate the activity of xylanase with final concentration of these metal ions 0.01 mol/L.[Conclusion]The screened strain Sp1 is of great value in application.

关 键 词：木聚糖酶 赭绿青霉 发酵条件 酶学特性 

分 类 号：S188[农业科学—农业基础科学]