黑曲霉产木聚糖酶的固态发酵条件优化  

Optimization of Culture Conditions for Xylanase Production by the Solid-state Fementation with Aspergillus niger

作　　者：周晨妍[1] 张金华[2] 马雪婷[1] 周锋[1] 周克楠[1] 丰慧根[1] 

机构地区：[1]新乡医学院生命科学技术系河南省医学遗传学与分子靶向药物高校重点实验室培育基地,河南新乡453003 [2]新乡医学院护理学院,河南新乡453003

出　　处：《安徽农业科学》2010年第21期11052-11054,共3页Journal of Anhui Agricultural Sciences

基　　金：新乡医学院基金项目(100794)资助

摘　　要：[目的]研究培养基组成和培养条件对黑曲霉固态发酵产木聚糖酶的影响。[方法]以木聚糖酶酶活为评价指标,利用单因素试验和L9(34)正交试验考察摇瓶发酵条件下培养基组成、pH值、培养时间、培养温度和接种量对黑曲霉产生木聚糖酶的影响。[结果]最佳培养基组成为:麸皮与玉米芯质量比5∶3,(NH4)2SO4、KH2PO4、CaCl2和MgSO4相对于固体料的质量百分数分别为1.0%、0.2%、0.1%和0.1%),料液比1∶1.7;最优培养条件为:pH值7.5、培养温度28℃、培养时间60h,其间翻曲2～3次;在此工艺条件下,木聚糖酶的活力可达14698.21IU/g。[结论]该优化条件为木聚糖酶的工业化生产和应用提供了依据。[Objective]To investigate the solid fermentation for xylanase production by Aspergillus niger.[Method] The single-factor tests and orthogonal experiment were employed to evaluate the optimal fermentation conditions for xylanase production by solid-state fermentation with Aspergillus niger.[Result]The optimum conditions for producing xylanase are as follows：the ratio of wheat bran：cob in medium 5∶3,（NH4）2SO4 1.0 %,KH2PO4 0.2%,CaCl2 0.1%,MgSO4 0.1%,the ratio of solid to fluid 1∶1.7,the inoculum 1.0%,the initial pH 7.5,the temperature 28 ℃,and the time for fermentation 60 h.Under those conditions,the enzyme activity of xylanase roduced by the strain reached 14 698.21 IU/g dry medium.[Conclusion]This study was meaningful for the production and application of xylanase in industry.

关 键 词：固态发酵 木聚糖酶 黑曲霉 正交试验 

分 类 号：Q814[生物学—生物工程]