邻苯二甲酸二异丁酯对小鼠抗氧化酶活性及DNA损伤影响的研究  

Effect of diisobutyl phthalate on antioxidse activity and DNA damage in mice

作　　者：马宁[1] 张文众[1] 冯永全[1] 徐海滨[1] 

机构地区：[1]中国疾病预防控制中心营养与食品安全所,北京100050

出　　处：《卫生研究》2010年第5期549-551,共3页Journal of Hygiene Research

基　　金：国家科技支撑计划课题(No2006BAK02A07)

摘　　要：目的对邻苯二甲酸二异丁酯(DiBP)引起机体组织氧化损伤进行初步研究,借此探讨邻苯二甲酸二异丁酯对自由基氧化损伤的作用机制。方法将60只昆明小鼠在实验动物房内适应3天,按体重随机分为5组:1个对照组和4个不同剂量的DiBP组(Ⅰ组:50mg/kg,Ⅱ组:250mg/kg,Ⅲ组:500mg/kg,Ⅳ组:1000mg/kg)。其中,对照组给予玉米油溶剂灌胃,而各DiBP组给予相应剂量的邻苯二甲酸二异丁酯玉米油溶液灌胃,实验连续进行8周,普通饲料喂养,自由饮水。实验结束后,拔眼球取血分离淋巴细胞,进行彗星实验;测定肝脏组织超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)的活性及丙二醛(MDA)、8-羟基脱氧鸟苷(8-OHdG)的含量。结果各DiBP组小鼠的肝SOD和GSH-Px酶活性比对照组显著较低(P<0.05);MDA含量在DiBPⅢ和Ⅳ组显著高于对照组(P<0.05),DiBPⅡ组的8-OHdG含量亦显著高于对照组(P<0.01);各DiBP组小鼠的DNA出现氧化损伤,与对照组相比,差异具有显著性(P<0.05)。结论在本实验条件下,邻苯二甲酸二异丁酯能够导致实验小鼠机体组织发生氧化应激,从而引起机体抗氧化系统酶活性的改变以及机体组织不同程度的氧化应激损伤。Objective To investigate the oxidative damage of mice induced by diisobutyl phthalate（DiBP） and the mechanism of free radical oxidative damage caused by DiBP.Methods Sixty KunMing mice were divided by weight into 5 groups after accommodation to the experimental animal room for 3 days.The control group and 4 DiBP groups,group Ⅰ,Ⅱ,Ⅲ and Ⅳ,were given DiBP in corn oil by gavages at the dosage of 0,50,250,500 and 1000mg/kg respectively.The mice were fed with normal diets and drinking water freely for 8 weeks.By the end of the experiment,the comet assay of blood and SOD,GSH-Px,MDA and 8-OHdG of liver were tested.Results The activities of SOD and GSH-Px in DiBP groups were significantly lower than the control group（P0.05）;the MDA contents of DiBP group Ⅲ and group Ⅳ were significantly higher than the control group（P0.05） and the 8-OHdG content of group Ⅱ was significantly higher than the control group（P0.01）.The comet assay showed that the oxidative damage of DNA in DiBP groups was significant in comparison with the control group（P0.05）.Conclusion Oxidative stress induced by diisobutyl phthalate can decrease the activities of antioxidative enzymes and result in oxidative damage of tissues.

关 键 词：邻苯二甲酸二异丁酯 8-羟基脱氧鸟苷 彗星实验 氧化应激 DNA损伤 

分 类 号：TS201.6[轻工技术与工程—食品科学] Q553[轻工技术与工程—食品科学与工程]