产志贺毒素突变株的毒力分析  

The virulence of Shiga toxin-producing Escherichia coli variants

作　　者：戴诗皎[1] 李睿[1] 刘志国[1] 宫本敬久[2] 

机构地区：[1]武汉工业学院生物与制药工程学院,湖北武汉430023 [2]日本九州大学农学部生命科学与技术学院,日本福冈8128581

出　　处：《中国酿造》2010年第10期145-147,共3页China Brewing

基　　金：湖北省自然科学基金重点项目(2009CDA118)

摘　　要：对2株食物中毒病人体内分离的产志贺毒素突变株EC130和EC169进行毒力分析。EC130和EC169携带stx基因但不能正常表达志贺毒素,具有eae基因和hly基因,仍具有一定毒力。初步探讨了产志贺毒素突变株不能正常表达志贺毒素的机理,高产志贺毒素的对照株携带Q933基因,而EC130和EC169不携带Q933基因。结果表明,单一采用志贺毒素作为检测靶标,容易造成产志贺毒素突变株漏检,今后在检测食品中产志贺毒素株时应增加检验eae基因和hly基因。The virulence of two Shiga toxin-producing Escherichia coil （STEC） variants （strains EC130 and EC169）isolated from food-borne patients were analyzed. EC130 and EC169 had stx gene but could not produce Shiga toxin. They contained both eae and hly genes, therefore they were still pathogenic. The reason for the non-expression of Shiga toxin of the two STEC variants was investigated. Control strains which produced high Shiga toxin was positive for Q933, while EC130 and EC169 were negative for Q933 by PCR determination. These results suggested that STEC variants might be missed if only detect Shiga toxin. For the rapid screening of STEC in food samples, eae and hlygenes should also be detected.

关 键 词：产志贺毒素突变株 毒力 食品检测 

分 类 号：R155.5[医药卫生—营养与食品卫生学]