紫外分光光度法测定啤酒中α-酸的含量  

Determination of α-Acid Content in Different Beers by Ultraviolet Spectrometry

作　　者：张海容[1] 任晶[1] 陈金娥[1] 王迎进[1] 

机构地区：[1]忻州师范学院生化分析技术研究所,山西忻州034000

出　　处：《分析科学学报》2010年第5期581-583,共3页Journal of Analytical Science

基　　金：山西省归国留学基金(No.2008-90);山西省国际科技合作项目(No.2010081020)

摘　　要：采用紫外分光光度法测定了8种不同品牌啤酒中α-酸的含量。实验考察了不同有机溶剂、介质酸度对α-酸紫外吸收的影响。结果表明:α-酸在279nm处有较强的吸收,方法线性范围在10～50μg/mL,检出限2.6μg/mL,相对标准偏差2.01%。8种不同品牌啤酒中α-酸的含量(μg/mL)为:百威啤酒(392.07)>蓝带啤酒(383.82)>崂山啤酒(373.72)>雪花啤酒(238.26)>司陶特黑啤酒(99.49)>燕京啤酒(55.16)>青岛啤酒(54.91)>哈尔滨啤酒(35.05),平均偏差小于5%。用标准加入法测定其回收率,样品回收率范围在93.81%～105.15%之间。The α-acid content in the 8 kinds of different beers was determined by ultraviolet spectrometry method.The effects of the different organic solvents and pH on the UV absorption of α-acid were investigated.The results showed that the absorption peak of α-acid was at 279 nm,the linear range,limit of detection and RSD were 10~50 μg/mL,2.6 μg/mL and 2.01% for α-acid,respectively.The α-acid content in 8 different kinds of beers was as follows（μg/mL）：Budwei（392.07）Blue Ribbon（383.82）Laoshan（373.72）Xuehua（238.26）Sitaotehei（99.49）Yanjing（55.16）Tsingtao（54.91）Harbin（35.05）with a mean deviation less than 5%.The method has been successfully applied to determine α-acids in different beers with recovery in the range of 93.81%~105.15%.

关 键 词：啤酒 Α-酸 紫外分光光度法 

分 类 号：O657.3[理学—分析化学]