豆腐柴叶提取低酯果胶的研究  

Low-ester Pectin Extraction from Premna Leaves

作　　者：廖雯娟[1] 蒋立科[1] 魏练平[1] 

机构地区：[1]安徽农业大学生命科学学院,安徽合肥230036

出　　处：《中国野生植物资源》2010年第5期39-43,共5页Chinese Wild Plant Resources

基　　金：安徽省科技厅医药项目赞助(9119701)

摘　　要：豆腐柴叶含有丰富的果胶。文中结合盐酸浸提法的基础上,采用醇氨降酯法提取豆腐柴叶的果胶并检测,提取率为部颁19.5%,pH值为2.9,水分和灰分分别为5.13%和8.67%,半乳糖含量为65%,酯化度为38.5%,所提取的低酯果胶的基本性质与国标检测标准接近。果胶成品经AB-8树脂柱纯化后,所提果胶的成分经硅胶板薄层层析初步测定,果胶多糖成分为葡萄糖,果糖,D-甘露糖3种,果胶的溶解度与温度呈正相关性,pH值对其溶解度影响不大。Premna leaves are rich in pectin.Based on hydrochloric acid extraction method,alcohol and ammonia ester lowering method was adopted to extract Premna leaves＇ pectin.The extraction rate was 19.5%,and the testing results showed that its pH value was 2.9;moisture,ash and galactose content 5.13%,8.67% and 65% respectively.After purified by AB-8 resin column,silica gel thin-layer chromatography showed that the pectin polysaccharides were composed of glucose,fructose and D-mannose.There existed a positive correlation between the pectin＇s solubility and temperature,pH value appearing little affect on its solubility,though.

关 键 词：豆腐柴 低酯果胶 酯化度 溶解度 

分 类 号：S567[农业科学—中草药栽培]