高效液相色谱法检测小麦粉中的偶氮甲酰胺方法的研究  

Determination of azodicarbonamide in wheat flour by high-performance liquid chromatography

作　　者：弓耀忠[1] 巩强[1] 张烨[1] 李静[1] 杨宝[1] 闫霞[1] 

机构地区：[1]山西省食品质量监督检验中心,太原030012

出　　处：《现代仪器》2010年第2期65-67,共3页Modern Instruments

摘　　要：小麦粉中偶氮甲酰胺(azodicardonamide,ADA)用丙酮提取,提取液氮吹浓缩后用5%二甲基亚矾的水溶液定容,并用正己烷脱脂。样品供高效液相色谱仪测定,外标法定量。方法线性范围是0～100mg/L(r^2=0.9979),定量下限为4mg/kg。在小麦粉基质粉中分别添加10.0mg/kg、50.0mg/kg两个水平的偶氮甲酰胺标准品,方法的回收率为84%～90%,相对标准偏差小于5%。The azodicarbonamide（ADA） in wheat flour was extracted with acetone.The textract was then concentrated under nitrogen and diluted to lmL with 5% dimethyl sulfoxide solution .After removed the lipid by hexane,the extract was analyzed by HPLC with external standard method.The method has a good linearity in the range of 0-100mg/L（r^2=0.9979）with the quantification limit of 4.0mg/kg. The recoveries of ADA from wheat flour spiked at two levels of 10.0-50.0mg/kg were in the range of 84%-92%,with the realative standard deviation less than 5%.

关 键 词：小麦粉 偶氮甲酰胺 高效液相色谱(HPLC) 

分 类 号：O657.72[理学—分析化学]