Coordinated regulation of active and repressive histone methylations by a dual-specificity histone demethylase ceKDM7A from Caenorhabditis elegans  

作　　者：Hanqing Lin Yiqin Wang Yanru Wang Feng Tian Pu Pu Yi Yu Hailei Mao Ying Yang Ping Wang Lulu Hu Yan Lin Yi Liu Yanhui Xu Charlie Degui Chen 

机构地区：[1]State Key Laboratory of Molecular Biology, Shanghai Key Laboratory of Molecular Andrology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yueyang Road, Shanghai 200031, China [2]Affiliated Hospital of Nantong University, Nantong 226001, China [3]School of Life Sciences, Fudan University, 220 HanDan Road, Shanghai 200433, China [4]Institutes of Biomedical Sciences, Fudan University, 130 Dong-An Road, Shanghai 200032, China

出　　处：《Cell Research》2010年第8期899-907,共9页细胞研究（英文版）

摘　　要：H3K9me2 and H3K27me2 are important epigenetic marks associated with transcription repression, while H3K4me3 is associated with transcription activation. It has been shown that active and repressive histone methylations distribute in a mutually exclusive manner, but the underlying mechanism was poorly understood. Here we identified ceKDM7A, a PHD （plant homeodomain）- and JmjC domain-containing protein, as a histone demethylase specific for H3K9me2 and H3K27me2. We further demonstrated that the PHD domain of ceKDM7A bound H3K4me3 and H3K4me3 co-localized with ceKDM7A at the genome-wide level. Disruption of the PHD domain binding to H3K4me3 reduced the demethylase activity in vivo, and loss of ceKDM7A reduced the expression of its associated target genes. These results indicate that ceKDM7A is recruited to the promoter to demethylate H3K9me2 and H3K27me2 and activate gene expression through the binding of the PHD domain to H3K4me3. Thus, our study identifies a dual-specificity histone demethylase and provides novel insights into the regulation of histone methylation.

关 键 词：KDM7A DEMETHYLASE H3K9me2 H3K27me2 PHD JMJC HISTONE methylation 

分 类 号：Q52[生物学—生物化学] S512.1[农业科学—作物学]