顶孢霉发酵液提取物清除自由基能力的研究  

Study on radical scavenging ability of fermentation broth extracts of Acremonium sp.

作　　者：程洁[1] 陈双林[1] 

机构地区：[1]江苏省微生物资源产业化工程技术研究中心,江苏省生物多样性与生物技术重点实验室,南京师范大学生命科学学院,江苏南京210046

出　　处：《食品工业科技》2010年第11期155-157,423,共4页Science and Technology of Food Industry

摘　　要：为了研究筛选出的具有抗氧化活性的2株顶孢霉的发酵液提取物清除自由基的能力,本研究制备了2株顶孢霉的发酵液提取物,比较了其清除O2-·、·OH和DPPH·三种自由基的能力。结果表明:2株顶孢霉发酵液提取物的相对还原力(即总抗氧化活性)均显著高于维生素E;对O2-·自由基的清除率也均高于维生素E,其中,菌株YX015更为高效,在1.0mg/mL浓度时对O2-·自由基的清除率达到49.15%;2菌株清除·OH和DPPH·的能力均显著弱于维生素E,但都高于其自身清除O2-·的能力,在浓度达到1.0mg/mL时,菌株CA022对·OH的清除率为60.43%;菌株YX015对·OH的清除率为83.33%。Fermentation broth extracts of 2 strains of Acremonium sp.was prepared.Their radical scavenging ability was studied and compared.The results showed that fermentation broth extracts of 2 strains of Acremonium sp.had stronger reducing power（ i.e.total antioxidant capacity）than Vitamin E.They also had greater abilities than Vitamin E in scavenging O2^-· .The strain YX015 was better and its scavenging rate was 49.15% at the concentration of 1.0mg/mL.The scavenging effects of the 2 strains on · OH and DPPH · was notably weaker than Vitamin E,but stronger than scavenging effects of themselves on O2^-·.At the concentration of 1.0mg/mL,the scavenging rate of the strain CA022 to · OH was 60.43% and the scavenging rate of the strain YX015 to · OH was 83.33%.

关 键 词：顶孢霉 抗氧化活性 自由基 

分 类 号：TS201.1[轻工技术与工程—食品科学]