耐胃液乳酸菌的筛选、鉴定与驯化  

Screening,identification and domestication of the tolerable gastric lactic acid bacteria

作　　者：柏建玲[1] 吴清平[1] 张菊梅[1] 莫树平[1] 胡文杰[1] 胡事君 

机构地区：[1]广东省微生物研究所,广东省菌种保藏与应用重点实验室,广东省微生物应用新技术公共实验室,广东广州510070 [2]东莞市农业科学研究中心,广东东莞523000

出　　处：《食品工业科技》2010年第11期190-192,195,共4页Science and Technology of Food Industry

基　　金：粤港关键领域重点突破项目招标项目(2007168603)

摘　　要：利用本实验室分离保存的乳酸菌菌种17株,经过耐胃液实验筛选,得到耐胃液能力较强、生长良好的乳酸菌菌株5株,选取4株菌株利用API乳酸菌生化系统和16S rDNA分析鉴定,并进行耐胃液驯化选育。结果表明,鉴定的4株乳酸菌中2株(编号:BR1-2和R8)为副干酪乳杆菌(Lactobacillus paracasei),1株(编号:BR3-2)为屎肠球菌(Enterococcus faecium),1株(编号:BR2-2)为乳酸片球菌(Pediococcus acidilactici);驯化后各菌株耐胃液能力均有不同程度的提高,其中BR1-2经人工胃液处理存活率提高52.2%;其他三株菌株的存活率也均提高20%以上。By gastric resistant test,5 gastric tolerable strains were screened from 17 strains of the conserved strains.With API lactic acid bacteria biochemistry system and 16S rDNA analysis,the gastric tolerable property of the 4 strains of the 5 strains were domesticated.The results showed that 2 strains of the 4 identified strains（BR1-2 and RS） were Lactobacillus paracaseL Another 2 strains, one was Enterococcus faecium, the last one was Pediococcus acidilactici.After the domestication,the gastric resistant ability of the 4 strains was higher than before. The survival rate of BR1-2 was advanced by 52.2% ,the other 3 strains＇ survival rates were advanced over 20%.

关 键 词：人工胃液 驯化 乳酸菌 耐胃液能力 

分 类 号：TS252.1[轻工技术与工程—农产品加工及贮藏工程]