草鱼鱼鳞中活性胶原蛋白提取工艺及参数优化  

Extraction Optimization of Collagen from Grass Carp Scales

作　　者：王艳[1] 汪海波[1] 桂萌[1] 王孟津[1] 张含俊[1] 刘良忠[1] 

机构地区：[1]武汉工业学院食品科学与工程学院,湖北武汉430023

出　　处：《食品科学》2010年第18期70-76,共7页Food Science

基　　金：湖北省自然科学基金重点项目(2009CDA117);武汉市科技局科技攻关计划项目(200920137006);武汉工业学院研究生创新性实验计划资助项目(ZD16)

摘　　要：以草鱼鱼鳞为原料,在低于蛋白变性温度的条件下提取酸溶性胶原蛋白(ASC)和酶溶性胶原蛋白(PSC),并对鱼鳞原料的前处理方法、胶原蛋白提取工艺进行优化。结果表明,在鱼鳞原料的前处理工艺中,以0.1mol/L的Na2CO3溶液为试剂脱除原料中杂蛋白的最佳工艺条件为室温、料液比1:40(g/mL),300r/min搅拌处理3次,每次6h;以0.3mol/L的EDTA为试剂脱除原料中矿物质杂质的最佳工艺条件为室温、料液比1:60,搅拌处理3次,每次12h;ASC的最佳提取工艺条件为:提取温度不高于25℃,料液比1:60,醋酸浓度0.8mol/L,提取3次,每次24h;PSC的最佳提取工艺条件为:胃蛋白酶用量为鱼鳞原料质量的3.0%、醋酸浓度0.8mol/L、料液比1:30、提取温度4℃、提取2次,每次24h。Acid-soluble collagen（ASC） and pepsin-soluble collagen（PSC） were extracted from the scales of grass carp at low temperature,and optimal extraction parameters were explored.The optimal parameters for removing impurity proteins from fish scales with 0.1 mol/L Na2CO3 were material/liquid ratio of 1：40 for mixing 3 times for 6 h each time at room temperature.The optimal parameters for removing mineral impurities with 0.3 mol/L EDTA were material/liquid ratio of 1：60 for mixing 3 times 12 h for each time at room temperature.The optimal parameters for ASC extraction were material/liquid ratio of 1：60 for extraction with 0.8 mol/L acetic acid 3 times for 24 h each time at 25 ℃.The optimal parameters for PSC extraction were amount of pepsin on the basis of the weight of fish scales,3.0%;acetic acid concentration,0.8 mol/L;and material/liquid ratio 1：30 for twice extraction at 4℃ for 24 h each time.

关 键 词：草鱼鱼鳞 胶原蛋白 提取 参数优化 

分 类 号：TS201.7[轻工技术与工程—食品科学]