气相色谱-质谱法测定蜂王浆中的植物甾醇组成  

Sterol Composition Analysis of Royal Jelly by Gas Chromatography Coupled with Mass Spectrometry

作　　者：徐响[1,2] 孙丽萍[1,2] 董捷[1,2] 李天骄[1] 穆雪峰[1] 沈新锋[1] 

机构地区：[1]中国农业科学院蜜蜂研究所,北京100093 [2]国家农产品加工技术研发中心蜂产品分中心,北京100093

出　　处：《食品科学》2010年第18期317-320,共4页Food Science

基　　金：农业部公益性行业(农业)科研专项(nyhyzx07-041);国家现代农业(蜂)产业技术体系建设专项(NYCYTX-43)

摘　　要：为探明蜂王浆中的植物甾醇组成,蜂王浆样品经冻干、萃取得到王浆脂类物质,再经过皂化、衍生后使用HP-5MS柱进行分离,采用GC-MS对蜂王浆中甾醇类物质进行鉴定,并用GC-FID内标法进行定量分析。结果表明:蜂王浆中除含有少量胆固醇外,还含有25-羟基-24-甲基胆甾醇、菜油甾醇、豆甾醇、β-谷甾醇、Δ5-燕麦甾醇、Δ7-燕麦甾醇等植物甾醇;蜂王浆中总甾醇含量在51mg/100g鲜王浆以上。其中,25-羟基-24-甲基胆甾醇、菜油甾醇、β-谷甾醇和Δ5-燕麦甾醇含量较高,分别占总甾醇含量的36.79%、6.30%、19.75%和27.99%。To figure out the sterol composition of royal jelly,royal jelly samples were lyophilized and extracted and the extracted lipid substances were then saponified,derivatized and separated on a HP-5MS column prior to gas chromatography with flame ionization detection（GC-FID） coupled with mass spectrometric identification of sterols,and quantitative analysis was finally conducted by internal standard method.A small amount of cholesterols was contained in royal jelly,and the main sterols were 25-hydroxy-24-methylcholesterol,campesterol,stigmasterol,β-sitosterol,Δ5-avenasterol and Δ7-avenasterol.The total content of sterols in 100 g of fresh royal jelly was higher than 51 mg.Among these sterols,the most predominant components were 25-hydroxy-24-methylcholesterol,campesterol,β-sitosterol and Δ5-avenasterol,accounting for 36.79%,6.30%,19.75% and 27.99%,respectively.

关 键 词：蜂王浆 植物甾醇 气相色谱-质谱法 

分 类 号：TS201.2[轻工技术与工程—食品科学]