绿衣观音土曲细菌DNA的提取  

DNA extraction of Green-covering Guanyin Tuqu

作　　者：李睿[1] 陈道玉 陈平[1] 许芳[1] 

机构地区：[1]武汉工业学院生物与制药工程学院,湖北武汉430023 [2]湖北劲牌有限公司,湖北大冶435100

出　　处：《中国酿造》2010年第11期127-128,共2页China Brewing

基　　金：湖北省自然科学基金重点课题(2009CDA118)

摘　　要：采用土壤DNA提取试剂盒和SDS-酶裂解法分别提取观音土曲的细菌DNA。基于SDS-酶法裂解原理的提取方法获得的结果比较理想,此方法获得的基因组片段大于15kb;A260/A280为1.82,A260/A230为1.54,DNA纯度很高;提取的DNA不经纯化能直接用于细菌16S rDNA的扩增。该法提取的DNA能直接用于后续的分子生物学实验操作。The bacterial DNA of Guanyin Tuqu was extracted by commercial soil DNA isolation kit and SDS-enzyme lysis method, respectively. Effect of method based on SDS-enzyme lysis was better than that of commercial isolation kit. Gene fragments obtained by the method were higher than 15 kb in size and ratios of A260/A280 and A260/A230 were 1.82, and 1.54 respectively. Purity of extracted DNA was sufficient for amplification by 16S rDNA PCR. The DNA extracted by SDS-enzyme lysis method could be directly used for further molecular experiments.

关 键 词：白酒 小曲 DNA提取 16S RDNA PCR 细菌 

分 类 号：Q939.9[生物学—微生物学]